摘要
目的探讨黄芪在体内对胰岛β细胞的保护作用。方法将56只健康雄性昆明小鼠随机分为对照组、糖尿病（DM）对照组及不同剂量黄芪干预组。不同剂量黄芪预处理小鼠，链脲佐菌素（STZ）腹腔注射诱导DM发生，观察DM发生情况；硝酸还原酶法检测各组小鼠血清中一氧化氮（NO）水平；化学比色法检测血清中诱导型一氧化氮合酶（iNOS）活性；ELISA法检测血清胰岛素水平；病理切片观察胰岛炎积分；末端脱氧核苷酰基转移酶介导dUTP切口末端标记法检测胰岛β细胞凋亡。结果1．DM对照组小鼠于STZ注射1周后开始发病。黄芪大剂量[30g/（kg·d）]组于STZ注射2周后开始发病，与DM对照组比较，DM发生延缓，DM发病率显著降低（P〈0．01）。黄芪小剂量[15g／（kg·d）]组于STZ注射1周后开始发病，与DM对照组比较，DM发病率有所降低，但差异无统计学意义（P〉0．05）。2．与DM对照组比较，黄芪大剂量组显著降低iNOS活性和NO水平（P均〈0．01），同时降低胰岛炎积分和胰岛β细胞凋亡率（P均〈0．01）。黄芪小剂量组上述指标均有所降低，但差异无统计学意义（P均〉0．05）。结论在体内黄芪对胰岛β细胞具有保护作用，与降低iNOS活性、减少NO生成，抑制胰岛β细胞凋亡有关；并可改善机体清除自由基能力，有效预防和延缓DM的发生。
Objective To investigate the protective effect of Astragalus on islet β cell apoptosis in vivo. Me- thods Fifty-six healthy male mice were divided into control group,diabetic mellitus（DM） group, and Astragalus pre- treatment group. After pretreatment with different doses of Astragalus, each group of mice received intraperitoneal injec- tion of Streptozotocin （STZ） in order to induce DM, and then the incidence of DM was observed. Serum nitric oxide （NO）was measured by the nitratase method, the activity of induced nitric oxide synthase（iNOS） was measured by chemical colorimetric method, and insulin level was detected by enzyme linked immunosorbent assay method. Insulitis score was evaluated according to pancreatic histology. Islet β cell apoptosis was measured by using a terminal dexynu- cleotidyl transferase （TdT）-mediated dUTP nick end labeling assay. Results 1. DM attack began 1 week after STZ in- jection in DM group. Pretreatment with 30 g/（ kg. d） of Astragalus, DM appeared 2 weeks after STZ injection. Com- pared with DM group, the onset of DM was delayed, and the incidence of DM was significantly reduced（ P 〈 0.01 ）. Af- ter pretreatment with 15 g/（ kg . d） of Astragalus, the DM began 1 week after STZ injection, compared with DM group, the incidence of DM was reduced, but there was no statistical difference （ P 〉 0.05 ）. 2. Compared with DM group, after pretreatment with 30 g/（ kg . d） of Astragalus, the activity of iN OS was significantly inhibited（ all P 〈 0.01 ）, and then NO level significantly declined （ all P 〈 0.01 ）, and the insulitis score and apoptosis of 13 cells were also significantly de- creased （ all P 〈 0.01 ） ; after pretreatment with 15 g/（ kg . d） of Astragalus, there was no statistical difference in all the indexes （ all P 〉 0.05）. Conclusions Astragalus can protect islet β cells of mice in vivo, which is associated with its inhibition on the iNOS activity, reduction on NO generation, and can decreaseβ cells insulit
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第8期577-580,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
国家自然科学基金（81170762）
山东省自然科学基金（Y2008C50）
关键词
黄芪
胰岛Β细胞
一氧化氮
糖尿病
凋亡
小鼠
Astragalus
Islet β cell
Nitric oxide
Diabetes mellitus
Apoptosis
Mouse
