摘要
目的:研究番茄红素对巨噬细胞源性荷脂细胞胆固醇代谢及其相关蛋白固醇调节元件结合蛋白1(Srebp-1)、小凹蛋白1(Caveolin-1)表达的影响。方法:建立巨噬细胞源性荷脂细胞模型,采用高效液相色谱法考察不同浓度(12.5、25、50μmol/L)番茄红素作用于荷脂细胞24h及25μmol/L番茄红素作用于荷脂细胞不同时间(12、24、48、72h)后细胞内总胆固醇(TC)、游离胆固醇(FC)和胆固醇酯(CE)的含量;蛋白印迹法检测番茄红素(25μmol/L、24h)组及其与Srebp-1蛋白抑制剂ALLN(20μmol/L、24h)联用组对荷脂细胞中Srebp-1、Caveolin-1蛋白表达的影响。另设立正常巨噬细胞组和荷脂细胞组进行比较。结果:与正常巨噬细胞组比较,荷脂细胞组细胞内TC、FC、CE含量均明显升高,Srebp-1、Caveolin-1蛋白表达明显减弱(P<0.05);与荷脂细胞组比较,番茄红素组荷脂细胞内TC、FC、CE含量均明显降低且呈一定的浓度和时间依赖性,Srebp-1、Caveolin-1蛋白表达明显增强(P<0.05);与番茄红素组比较,联用组细胞内Srebp-1、Caveolin-1蛋白表达均明显减弱(P<0.05)。结论:番茄红素能引起巨噬细胞源性荷脂细胞内TC、FC、CE含量降低,且可能与Srebp-1及Caveolin-1蛋白的表达上调有一定关系。
OBJECTIVE：To study the effects of lycopene on the cholesterol metabolism and its related protein Srebp-1,Caveolin-1 expression in macrophage-derived cholesterol-loaded cells.METHODS：Macrophage-derived cholesterol-load cell model was established.The contents of total cholesterol（TC）,free cholesterol（FC）and cholesterd esterase（CE）were determined by HPLC after cholesterol-load cells were treated with different concentrations of lycopene（12.5,25 and 50 μmol/L）for 24 h or treated by 25 μmol/L lycopene for different time（12,24,48,72 h）;Western blotting assay was used to detect the effects of lycopene 25 μmol/L or combined with ALLN 20 μmol/L on the expressions of Srebp-1,Caveolin-1;above index were compared with normal macrophage group and cholesterol-loaded cell group.RESULTS：Compared with normal macrophage group,the contents of TC,FC and CE were increased significantly in cholesterol-loaded cell group,while protein expression of Srebp-1,Caveolin-1 were decreased significantly（P0.05）;compared with cholesterol-loaded cell group,lycopene induced the decrease of TC,FC and CE content in dose and time-dependant manner and enhanced the protein expression of Srebp-1,Caveolin-1（P0.05）.Compared with lycopene group,the protein expression of Srebp-1,Caveolin-1 were decreased significantly in ALLN combination group（P0.05）.CONCLUSIONS：Lycopene can induce the decrease of TC,FC and CE content in macrophage-derived cholesterol-road cells,which may be associated with the up-regulation of protein expression of Srebp-1,Caveolin-1.
出处
《中国药房》
CAS
CSCD
2013年第21期1942-1944,共3页
China Pharmacy
基金
衡阳市2012年科学技术发展计划项目(No.2012KJ8)
