摘要
目的探讨脂联素基因rs2241766(T45G)和rs15011299(G276T)多态性位点(SNP)与中国大城市汉族老年人代谢综合征(MS)的相关性。方法根据国际糖尿病联盟(IDF)的MS定义,从2002年营养与健康状况调查对象中选取大城市60岁以上汉族老年人,分为MS组和正常对照组,共907人。检测这些研究对象血浆脂联素和胰岛素浓度,提取全血基因组DNA,采用Taqman方法检测所有研究对象脂联素基因rs2241766(T45G)和rs15011299(G276T)位点的基因型。并分析各SNP位点基因型与MS的相关性。结果 rs15011299的三种基因型在MS组和正常对照组间的总体分布差异有显著性,MS组T等位基因频率显著高于正常对照组。在rs15011299位点,GG、GT、TT三组基因型的研究对象的BMI、脂联素浓度差异有显著性,GG+GT型研究对象的BMI、腰围、血浆胰岛素浓度显著低于TT型研究对象,而血浆脂联素浓度显著高于TT型研究对象;在rs2241766位点,TT、TG、GG三组基因型的研究对象间BMI及舒张压差异有显著性,TT型研究对象BMI、腰围及舒张压显著高于TG+GG型研究对象。logistic回归分析显示rs15011299TT型研究对象与GG型研究对象相比发生MS的风险高,其校正的OR值为3.19(1.31,7.78)。结论脂联素基因rs15011299位点的变异可能与MS相关,G→T突变可能增加MS的发生,使腰围、BMI增加,并降低血浆脂联素水平。
Objective To investigate the association of adiponectin gene rs2241766（T45G）and rs15011299（G276T）single nucleotide polymorphrism（SNP） with metabolic syndrome（MS） in older han adults from major cities in China.Methods A total of 907 older Han adults were selected from 18 major cities of the China National Nutrition and Health Survey in 2002.According to the MS definition proposed by International Diabetes Federation,the subjects were divided into MS and control groups.Plasma adiponectin and insulin concentrations were measured.Genomic DNA was isolated from blood and genotypes of rs2241766 and rs15011299 were identified by Taqman method.Association of genotypes of adiponectin gene SNPs with MS was analyzed.Results There was significant difference in the distribution of genotypes of rs15011299 SNP between the MS and control group.The T allele frequency of rs15011299 was significantly increased in MS group.For rs15011299 site,there were significant differences of BMI and adiponectin level among the subjects carried GG,GT or TT genotypes.The GG＋GT carriers had lower BMI,waist circumference,insulin level and higher adiponectin level than those of TT carriers.For rs2241766 site,there were significant differences of BMI and diastolic blood pressure（DBP） among the subjects carried TT,TG or GG genotypes.The TT carriers had higher BMI,waist circumference and DBP than those of GG＋GT carriers.The TT carriers in rs15011299 had higher risk for MS than GG carriers（OR for TT v.s.GG=3.19;95%CI 1.31-7.78）.No association was found in rs2241766 with MS.Conclusion The adiponectin gene rs15011299 polymorphrism may be associated with pathogenesis of MS in older Han adults.G→T variance may be associated with an increased risk of MS and result in a decreased level of aiponectin and increments of waist circumference and BMI.
出处
《卫生研究》
CAS
CSCD
北大核心
2013年第3期353-359,共7页
Journal of Hygiene Research
基金
国家自然科学基金资助(No.30671750)
关键词
脂联素
单核苷酸多态性
代谢综合征
中国老年人
adiponectin
single nucleotide polymorphrism
metabolic syndrome
older Chinese adults
