摘要
目的:建立琥乙红霉素静脉乳剂的含量测定方法。方法:采用Agilent Zorbax SB-C_(18)柱(250 mm×4,6 mm,5μm),流动相为乙腈-0.02 mol·L^(-1)醋酸铵缓冲盐溶液(65:35),检测波长215 nm,流速为1.0 ml·min^(-1),柱温25℃,进样量20μl。结果:琥乙红霉素在60～600μg·ml^(-1)的浓度范围内与峰面积呈良好的线性关系(r=0.999 1),平均回收率为97.92%,RSD为0.43%(n=9)。结论:该方法简便、快速、准确,可用于琥乙红霉素的含量测定及稳定性分析。
Objective：To establish an HPLC method for the determination of erythromycin ethylsuccinate intravenous emulsions. Method：An Agilent Zorbax SB-C_（18） column（250 mm×4.6 mm,5μm） was used.The mobile phase consisted of acetonitrile-0.02 mol·L（-1） ammonium acetate solution（65：35） and the detection wavelength was 215 nm.The flow rate was 1.0 ml·min（-1）,the column temperature was 25℃and the injection volume was 20μl.Result：The linearity between the concentrations and the peak areas was obtained within the range of 60-600μg·ml（-1）（r =0.999 1）.The average recovery was 97.92%（RSD = 0.43%,n = 9）.Conclusion： The proposed method is simple,rapid and accurate,and suitable for the content and stability study of erythromycin ethylsuccinate intravenous emulsions.
出处
《中国药师》
CAS
2013年第5期675-676,共2页
China Pharmacist
