摘要
目的:拟建立一次性大强度耐力运动动物模型,观察AMPK活性变化对Akt-mTOR信号通路的影响。方法:36只SD大鼠进行1次跑台运动,坡度5%,运动强度25 m/min,运动时间60 min。取样为运动前、运动0.5 h、运动1 h、运动后1 h、运动后2 h、运动后6 h等6个点,每个采样点6只大鼠。采用同位素技术测定腓肠肌中AMPK活性的变化,采用Western blot方法,测定Akt、mTOR总蛋白含量及其磷酸化变化。结果:1)与安静组相比,运动各组AMPK活性都升高,有显著性或非常显著性差异(P<0.05或P<0.01),AMPK活性在运动0.5 h后开始升高,运动后1 h达到最高,运动后6 h开始下降但还高于安静组;2)与安静组相比,运动各组Akt、mTOR总蛋白含量没有显著性差异;3)与安静组相比,AktSer473磷酸化在运动0.5 h组显著升高(P<0.05),在运动1 h组非常显著性升高(P<0.01),而在运动后1 h组有下降趋势,但没有显著性差异,在运动后2 h、6 h组非常显著性下降(P<0.01);4)与安静组相比,mTORSer2448磷酸化在运动0.5 h,1 h组显著性升高(P<0.05);而在运动后1 h、2 h、6 h组mTORSer2448磷酸化显著性下降(P<0.05)。结论:1)AMPK活性升高,可能通过降低AktSer473磷酸化,抑制Akt-mTOR信号通路。2)一次性大强度耐力运动中,AktSer473磷酸化除受AMPK调节外,可能还受其它因素调节。
Objective ： The authors have probed into the effects of AMPK activation on Akt-mTOR signal pathway a- bout protein synthesis in rat skeletal muscle through the one-time high intensity exercise. Methods： 36 SD rats do a one-time treadmill exercise at a slope of 5% , an exercise intensity of 25 m/min, and an exercise time of 60 rain. The sampling times are different before exercise, after 0.5 h, 1 h of exercise, 1 h after exercise, 2 h after exercise and 6 h after exercise. The authors measured the changes of AMPK activity in gastrocnemius muscle by u- sing isotope technology. Phosphorylation mTORs^ser2448 was determined by western blot, as well as total Akt, total mTOR,phosphorylation Akts^ser473. Results： 1 ） The AMPK activity started to increase after 0. 5 h of exercise, reached its peak at 1 h after exercise, started to lower at 6 h after exercise but was still higher than that of rats in the control group ;2）In the course of exercise, Akt, roTOR total protein content, among the groups there were no significant differences. 3 ） Compared with those of rats in the control group, Akts^ser473 phosphorylation of rats in 0.5 h and 1 h exercise groups increased respectively, and the differences were significant or very significant （P 〈 0.05, P 〈0.01 ） ; compared with those of rats in the control group, AktS^ser473 phosphorylation of rats in the 2 h and 6 h af- ter exercise group decreased, and the differences were very significant （P 〈 0.01 ）. 4） Compared with those of rats in the control group, mTOR^ser448 phosphorylation of rats in 0.5 h and 1 h exercise groups increased respectively, and the differences were significant （P 〈 0.05 ） ; Compared with those of rats in the control group, mTOR^ser448 phospho-rylation of rats in the 1 h,2 h and 6 h after exercise group decreased, and the differences were significant （ P 〈 0.05）. Conclusion： 1 ） The activated AMPK may inhibit the Akt-mTOR signal pathway through decreasing Akt phosphorylation. 2） During one-time high intensity e
出处
《北京体育大学学报》
CSSCI
北大核心
2013年第4期53-57,共5页
Journal of Beijing Sport University
基金
国家自然科学基金(30971411)
河南科技厅课题(122300410257)
