摘要
目的拟通过使用WB对隐性梅毒TPPA/TP-ELISA检测结果不一致标本的检测比较,探讨WB对于隐性梅毒抗体检测结果的确证价值。方法 1.先用TPPA或TP-ELISA检测血清或血浆标本,对阳性标本分别交叉使用另一种方法复核。对两种方法检测结果不一致的60份标本,再用WB进行检测。2.对NCCL提供的梅毒标准血清盘,使用TP-ELISA和TPPA同时检测,WB结果采用NCCL提供。结果 1.用TP-WB对60例TPPA/TP-ELISA检测不一致的标本进行检测,51份TP-ELISA阳性,TPPA阴性的标本,WB检测结果是32份阳性,9份不确定,10份阴性;9份TP-ELISA阴性,TPPA阳性的标本,WB检测结果是8例阳性,1例可疑。2.血清盘40份标本,WB阳性26例,阴性14例,26例阳性标本分别用TPPA和ELISA检测,TPPA阳性24例,阴性2例;TP-ELISA检测26例全部阳性。而14例WB检测阴性标本分别用两种方法检测时,TPPA与之完全相符,TPELISA出现两例阳性。结论 WB可作为潜伏期梅毒检测TPPA、TP-ELISA二者结果不致时的确认方法。
Objective Confirmatory tests for latent syphilis have been carried out on samples with discordant re- sults when tested by TPPA and TP - ELISA to evaluate the value of WB method in latent syphilis test. MethodsThe serum or plasma samples of latent syphilis are tested by TPPA or TP - ELISA first, and the positive samples are retested by another method. A total of 60 samples with discordant results in these tests are tested by WB. TPPA and TP- ELISA are used to test the standard serum panel supplied by NCCL, and the WB test results are from NCCL. Results 51 samples with TP - ELISA positive TPPA negative and 9 with TP - ELISA negative TPPA posi- tive are tested by WB method. 32 samples from the 51 samples are positive, 9 uncertain and 10 negative when test- ed by WB. 8 samples are positive and 1 negative in the 9 samples when tested by WB. There were 26 positive and 14 negative samples in the 40 samples from the standard serum panel. 24 samples are positive and 2 negative when using TPPA to test the 26 positive samples. 26 positive samples are identified as positive by TP - ELISA. 12 sam- ples are negative and 2 positive when using TP- ELISA to test the 14 negative samples. And all negative samples are correctly identified by TPPA. Conclusion WB can be an accurate confirmatory test for latent syphilis samples when the testing results are discordant by TPPA and TP - ELISA tests.
出处
《口岸卫生控制》
2013年第2期20-23,共4页
Port Health Control
基金
国家质量监督检验检疫总局2008IK207
