摘要
【目的】为了研究FMO酶编码基因在草莓生长发育过程中的可能功能,【方法】以二倍体森林草莓‘黑龙江3号’为材料,基于八倍体栽培草莓‘久香’中已克隆基因序列,利用同源克隆技术获得了2个FMO酶编码基因:FvYUC1和FvYUC2基因;并运用DNAMAN软件和RT-PCR技术,分析比较了同源YUC基因在这两种倍性草莓中的分子特征和表达模式特征。【结果】同源性分析结果表明:在两种倍性草莓中YUC1氨基酸序列的一致性为99.31%,YUC2为99.02%;FvYUC1和FvYUC2之间的序列一致性为60.82%。定量RT-PCR分析表明,YUC1和YUC2在栽培草莓和森林草莓的各个组织中均表达,但表达模式差异很大,YUC1的优势表达组织在森林草莓中是幼叶、在栽培草莓中是根;而YUC2在森林草莓中的优势表达组织是匍匐茎,在栽培草莓中是小绿果。【结论】森林草莓和栽培草莓中存在高度保守的YUC同源基因,但它们在这两种倍性草莓中的主要功能可能有较大变异,将森林草莓功能基因研究结果推导至栽培草莓必须谨慎。
[Objective]The study was to elucmate me putat, vc ,, ,o （FMOs） related genes in the strawberry. [Method]Two YUC-type FMO genes FvYUC1-2 were isolated from woodland strawberry （Fragaria vesca var. Heilongjiang3#） based on a homologous cloning strategy, and the molecular characteristics and expression patterns of YUC homologous genes were comparatively analyzed in octoploid and diploid strawberries by using DNAMAN software and RT-PCR. [Result]The re- suits of homologous analysis showed that the sequence identity of YUC gene between these two strawber- ries was 99.31% for YUC1 at amino acid level, and 99.02% for YUC2, respectively. However, FvYUCI shared only 61.6% sequence identity with FvYUC2. Quantitative RT-PCR revealed that although YUC1 and YUC2 expressed in all tested six tissues of ＇Jiuxiang＇ and ＇Heilongjiang3#＇, their expression pat- terns differed greatly. YUCI was predominantly expressed in the young leaves of woodland strawberry, while in the roots of cultivated strawberry. YUC2 was preferentially expressed in the stolon of woodland strawberry, while in the small green fruit of cultivated strawberry. [Conclusion]These results suggested
出处
《果树学报》
CAS
CSCD
北大核心
2013年第3期390-396,共7页
Journal of Fruit Science
基金
上海农科院科技发展基金(农科发2012(13))
上海市科技兴农重点攻关项目(沪农科攻字(2012)第1-3号)
上海市科委自然基金(10ZR1426700)
上海市科委基础重点项目(12391901400)
农业部公益性行业(农业)科研专项(201003064)
关键词
森林草莓
生长素合成
同源克隆
表达模式
Fragaria vesca
Auxin biosynthesis
Homologous cloning
Expression pattern
