摘要
MTT法检测新鱼腥草素钠对脾细胞的增殖反应,胸腺细胞增殖法检测新鱼腥草素钠对脾细胞分泌IL-2的影响。在脾细胞培养液含有2.5mg/L ConA的条件下,128及256mg/L新鱼腥草素钠能够促进脾细胞增殖,与2.5mg/L ConA组对照,经统计学检验,差异有显著性(P<0.01)。64、128及256mg/L新鱼腥草素钠表现出提高脾细胞培养透析液中的IL-2水平的作用,与2.5mg/L ConA组比较,经统计学检验,差异有显著性(P<0.05或P<0.01)。
The action of sodium new houttuyfonate on the splenocyte proliferation was tested by MTT spectrophotometry,and the activity of sodium new houttuyfonate on the production of IL-2 from splenocytewas was investigated by measuring the proliferation of thymoeytes promoted by IL-2 from splenocyte. In condition that splenocyte culture solution contained 2.5 mg/L ConA,the proliferation and the level of IL-2 of splenocyte could be increased and promoted by 128 mg/L and 256 mg/L sodium new houttuyfonate. Compared with 2.5 mg/L ConA group,sodium new houttuyfonate groups have significant difference（P〈0.05or P〈0.01）.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第5期727-729,737,共4页
Chinese Journal of Veterinary Science
基金
国家公益性行业(农业)科技专项经费资助项目(201203040)
