摘要
目的探讨阿托伐他汀对大鼠脑出血后血肿周围组织细胞凋亡和细胞色素C（Cytochrome c，CytC）表达的影响。方法108只雄性Sprague—Dawley大鼠随机分为假手术组、生理盐水对照组和阿托伐他汀组（每组36只），各组再分为6h、12h、1d、3d、5d和7d时间点（每个时间点6只）。采用改良二次注血法制作脑出血模型。阿托伐他汀组在模型制作后给予阿托伐他汀灌胃（20mg／kg，1次／d），生理盐水对照组给予等体积生理盐水。采用行为学评价进行神经功能评分，TUNEL染色法检测血肿周围细胞凋亡，免疫组织化学法检测血肿周围组织CytC表达。结果行为学评价显示，阿托伐他汀组和生理盐水对照组神经功能评分均随着时间的推移而逐渐下降，6h、12h、1d和3d时无显著性差异，但5d[（0．50±0．55）分对（1．50±0．55）分；t=3．162，P：0．010]和7d[（0．17±0．41）分对（1．00±0．63）分；t=2．712，P=0．022]时阿托伐他汀组神经功能评分显著性低于生理盐水对照组。TUNEL染色法显示，生理盐水对照组与阿托伐他汀组凋亡细胞数量均先增加后减少，高峰出现在模型制作后1d时。各组间相同时间点血肿周围凋亡细胞数量均存在显著性差异（P均=0．000），且生理盐水对照组显著性多于假手术组和阿托伐他汀组（P均〈0．05），但在7d时阿托伐他汀组凋亡细胞数量与假手术组无显著性差异[（12．69±3．35）个对（9．33±2．07）个；P=0．148]。免疫组织化学法显示，生理盐水对照组与阿托伐他汀组CytC阳性细胞数量均先增加后减少，生理盐水对照组高峰出现在模型制作后12h时[（68．19±11．93）个]，而阿托伐他汀组出现在模型制作后1d时[（35．64±9．12）个]。各组问相同时间点血肿周围cytc阳性细胞数量均存在显著性差异（P均=0．000），且生理盐水对照组显著性多于假手术组和阿托伐他汀
Objective To investigate the effect of atorvastatin on cytochrome c （CytC） expression and neuronal apoptosis after intracerebral hemorrhage in rats. Methods A total of 108 male Sprague-Dawley rats were randomly allocated into 3 groups： sham operation group, saline control group, and atorvastatin group （n = 36 each group）. All the groups were redivided into 6 h, 12 h, day 1, 3, 5 and 7 time points （n =6 at each time point）. An intracerebral hemorrhage model was induced by using a modified two-step injection method. After modeling, atorvastatin was used for gavages （20 mg/kg, once a day） in the atorvastatin group. The saline control group was given the same volume of saline. Behavior evaluation was used for neurological score. TUNEL staining was used to detect apoptosis in perihematoma tissue. Immunohistochemical method was used to detect the CytC expression in perihematoma tissue. Results Behavior evaluation showed that the neurological scores decreased gradually with the passage of time in the atorvastatin group and the saline control group. There were no significant differences at 6 h, 12 h, day 1 and day 3, but the neurological scores in the atorvastatin group were significantly lower than those in the saline control group at day 5 （0. 50 ± 0. 55 vs. 1.50 ＋ 0. 55; t = 3. 162, P =0. 010） and day 7 （1.00 ±0. 63; t =2. 712, P =0. 022）. TUNEL staining showed that the numbers of apoptotic cells increased first and then decreased in the saline control group and the atorvastatin group. They reached the peak at 1 hour after modeling There were significant differences in the number of apoptotic cells in each group in perihematoma tissue at the same time point （all P = 0. 000）, and the significance in the saline control group was more than that in the sham operation group and the atorvastatin group （all P 〈0. 05）, but at day 7, there was no significant difference in the number of apoptotic cells between the atorvastatin group and the sham operation group （12. 69 ± 3.35 vs. 9. 33
出处
《国际脑血管病杂志》
北大核心
2013年第3期191-196,共6页
International Journal of Cerebrovascular Diseases
