摘要
建立环介导恒温扩增(LAMP)-石英晶体微天平(QCM)原位快速检测核酸的方法。将环介导恒温核酸扩增(LAMP)技术与石英晶体微天平(QCM)技术相结合,采用巯基化试剂分子组装方法,将LAMP反应体系中的4个引物之一固定于QCM电极上,在安装所述电极的QCM检测池中配置LAMP反应体系并进行环介导恒温核酸扩增,用QCM仪器在线原位检测频率变化,判断LAMP反应是否发生,进而判断体系中是否存在目标核酸特异基因。该方法检测核酸特异性强、灵敏度高,并且操作简便,有望发展成为快速筛查检测核酸的有效手段。
The objective of the study is to develop a loop-mediated isothermal amplification（ LAMP）-quartz crystal microbalance （QCM） method for rapid detection of a nucleic acid. The quartz crystal microbalance （ QCM ） combined with the loop-mediated iso- thermal amplification（LAMP） technology. Use the thiol reagent molecules assembly method to fix one of the four primers in the loop- mediated isothermal amplification reaction system on the QCM electrode, and establish the LAMP reaction system in the QCM detec- tion pool which the described electrode installed to proceed loop-mediated isothermal amplification. We use QCM instrument to test frequency changes online and determine whether the LAMP reaction occurs, thereby judging if the nucleic acid exist in the sys- tem. These results suggested that detection of nucleic acid by this method is an effective with high specificity and sensitivity. This assay is expected to become a valuable tool for rapid detection and identification of nucleic acid.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2013年第5期748-750,共3页
Chemical Research and Application
