摘要
目的 :研究β1 肾上腺素受体抗体在扩张性心肌病发病中可能的作用。方法 :将 14只新西兰兔随机分为两组 ,免疫组和对照组。β1 受体肽段作为抗原 ,给予免疫组家兔每月一次皮下注射 ,免疫时程为 1a。对照组除用生理盐水替代抗原外 ,注射过程同免疫组。 EL ISA法测定家兔血清中 β1 受体抗体效价。 1a后取两组动物心肌电镜下观察。结果 :免疫前两组动物及免疫后对照组动物β1 受体抗体效价均很低 ,而免疫后免疫组动物效价明显增高。免疫 1a后对照组动物心脏电镜下无异常表现 ;免疫组示心脏有坏死灶、心肌纤维缺失、过度收缩带出现等。结论 :β1
Objective:To investigate whether β 1-adrenoceptor antibody play a role in pathogenesis of dilated cardiomyopathy.Method:Fourteen Newzealands’rabbits were divided into two groups,the immunized group and the control group.Rabbits of immunized group were immunized with synthetic peptide corresponding to the second extracelluar loop of β 1-adrenoceptor and adjuvant monthly for one year.Control rabbits received the mixture with the same procedure as described above except a substitution of saline for corresponding peptide.All rabbits were bled to assay the titers of β 1-adrenoceptor antibody once a month using ELISA.After one year,their heart tissue was taken to do morphological examinations.Result:In sera from immunized rabbits,high titers of antipeptide antibody were found thougout the immunination period,whereas those of control group and immunized group before immunination were very low.Under electron microscopy,the hearts of immunized group showed focal myofibrillar lysis,neerosis and the loss of normal myofilament banding pattern with hypercontration bands.No alternative changes were found in control group.Conclusion:β 1-adrenoceptor antibody may play a role in pathogenesis of dilated cardiomyopathy.
出处
《山西临床医药》
2000年第6期428-429,共2页
Shanxi Clinical Medicine
