摘要
植物DNA甲基化酶对建立与维持基因组甲基化,调控基因表达,确保植物快速应对多种逆境具有重要作用。本研究利用多种生物信息学网站和软件初步明确了大豆中四类DNA甲基化酶蛋白及相应基因序列,并分析了进化关系和基因拷贝数,确定了基因在染色体上的物理位置及酶蛋白的理化性质与亚细胞定位,同时揭示了大豆DNA甲基化酶蛋白的结构域和组织表达部位特异性。这些研究结果有助于全面认知大豆DNA甲基化酶蛋白的特征对从遗传学上展开深入研究和剖析具有重要的理论指导作用。
DNA methylase in plants plays an important role on maintaining genome DNA methylation, regulating gene expression and prompt response to varied adverse circumstances. Many bioinformatics websites and softwares were employed in this paper. Four kinds of DNA methylases and corresponding gene orders were determined preliminarily in soybean. Their evolutionary relationships and gene copy numbers were analyzed in this study. The locations of all genes on the chromosomes and the physio-chemical property and subcellular localization of DNA methylases were identified. The domains of these proteins and tissue-specificity expression were identified. These results provided a guidline in further understanding the characteristics of soybean DNA methylases and their genetics.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2013年第2期225-230,共6页
Chinese Journal of Oil Crop Sciences
基金
黑龙江省教育厅科学技术研究(12521381)
国家自然科学基金项目(31201163)
