摘要
目的探讨人参皂苷Rh2对实验性SD大鼠子宫内膜异位症模型的抑制作用及其机制。方法外科手术法建立SD大鼠子宫内膜异位症模型,在第2次开腹后按模型异位内膜大小随机分成5组,分别是模型对照组(0.5%羧甲基纤维素钠,0.5%CMC-Na),孕三烯酮组(1.5 mg.kg-1)和Rh2低、中、高剂量组(5、10、40 mg.kg-1);连续灌胃给药21 d后,在末次给药24 h内麻醉解剖大鼠、采血及保存异位内膜组织。HE染色观察异位内膜病理组织学改变;Elisa测定血清中雌激素(Estrogen,E2)、孕激素(Progesterone,P)和抗子宫内膜抗体(Endometrial antibody,EMAb)水平;Western blot检测异位内膜组织中Bcl-2、Bax、Caspase-9、Caspase-3蛋白的表达情况。结果 Rh2(40 mg.kg-1)异位内膜组织明显萎缩,腺体数目明显减少,腺腔萎缩,腔内积液体积减少;Rh2(5、10、40 mg.kg-1)降低E2、P和EMAb的水平;Rh2(5、10、40 mg.kg-1)异位内膜组织中Bax/Bcl-2、Caspase-9和Caspase-3蛋白表达明显升高。结论 Rh2(40 mg.kg-1)可明显抑制实验性SD大鼠异位内膜生长,其作用机制可能与降低E2、P和EMAb水平、上调Bax/Bcl-2蛋白表达、激活细胞凋亡的内源性途径启动因子Caspase-9蛋白、激活执行因子Caspase-3蛋白表达有关。
Aim To investigate the effect of Ginsenoside Rh2 on the growth of ectopic endometrium in rat model of endometriosis and its mechanism of action.Methods The rat model of endometriosis was established by transplantation surgery. The model rats were randomly divided into five groups： model control group （0.5% CMC-Na）, gestrinone （1.5 mg ·kg-1） group, and Rh2 low-, medium- and high-dose （5,10, dO mg ·kg-l） groups. At the end of three weeks＇ treatment, the rats were sacrificed, and serum and ectopic endometrium were collected Histopathologic changes of ectopic endometrium were tested by HE staining. The levels of estrogen （ E2 ）, progesterone （P） and endometrial antibody （EMAb） were observed by ELISA method. The expression of apoptotic factors were tested by western blotting. Results Rh2 （40 mg ·kg-1） obviously suppressed the ectopic endometrium, and reduced the numbers of endometrial gland and the volume of glandular cavity. Rh2 （5,10,40 mg · kg-1 ） reduced dose-dependently the level of E2, P and EMAb, and increased the expression of Caspase-9, Caspase-3 and Bax/Bel-2. Conclusion Ginsenoside Rh2 （40 mg· kg-1） obviously inhibits the growth of ectopic endometrium in the established rat model of endometriosis, and its mechanism of action may relate to reducing the level of E2,P and EMAb, and increasing the protein expression of Caspase-9, Caspase-3 and Bax/Bcl-2.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2013年第5期718-723,共6页
Chinese Pharmacological Bulletin
