摘要
目的水杨酸甲酯糖苷(DL0309)是来源于民族药滇白珠的新型非甾体抗炎药,本实验的主要目的是评价其对角叉菜胶致大鼠胸膜炎模型的抗炎作用及其可能的机制。方法将48只♂SD大鼠按体重随机分为正常对照组、模型对照组、阳性对照组(地塞米松)、DL0309低、中、高剂量组。通过注射角叉菜胶,建立大鼠急性胸膜炎模型。造模后5 h处死大鼠,通过测定胸腔渗出液的体积,对渗出液中的白细胞计数,并测定渗出液中蛋白质的含量来观察药物对该模型的抗炎作用;通过测定渗出液中一氧化氮(NO)、肿瘤坏死因子(TNF-α)、白介素1β(IL-1β)和前列腺素E2(PGE2)以及血浆中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,来考察DL0309对该模型的抗炎作用机制。结果结果显示地塞米松和DL0309均可明显降低胸膜炎大鼠胸腔炎性渗出液的体积以及渗出液中的白细胞数量和蛋白质含量,同时对于胸腔炎性渗出液中的NO、TNF-α、IL-1β和PGE2的含量均有不同程度的抑制,能不同程度地降低血浆中MDA含量,升高SOD活力。结论 DL0309具有抗角叉菜胶诱导的大鼠急性胸膜炎作用。
Aim To study the anti-inflammatory effect and mechanism of methyl salicylate glycosides （DL0309） in carrageenan-induced acute pleurisy model in rats, and to provide the pharmacology mechanism for the effects and clinical use of DL0309. Methods Forty-eight male rats were divided into 6 groups： normal group, pleurisy model group, dexamethasone group （positive drug）, DL0309 low dose （150 mg· kg^- 1） , medium dose （300 mg· kg^- 1 ） , and high dose （600mg· kg^- 1） groups. The acute pleurisy model was established by injecting carrageenan into chest. The rats were sacrificed after 5 h and the blood and the exudates were collected. The ELISA method was used to detect the production of TNF-α, IL-1β, PGE2 in exudates. The NO content was measured by Griess assay. The contents of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） were detected by kit instruction. Results Compared with acute pleurisy model group, DL0309 decreased the volume of exudates, the total leukocyte numbers and protein contents in exudates. Meanwhile, D133309 could significantly reduce the levels of IL-1β and PGE2 and decrease the contents of TNF-α and NO. In addition, DL0309 was also able to decrease the contents of MDA and the activity of SOD in plasma in carrageenan-treated rats. Conclusion D133309 has potent anti-inflammatory effects on carrageenan-induced acute pleurisy model in rats.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2013年第3期328-332,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81073120)
