摘要
目的:检测与分析溃疡性结肠炎(UC)患者外周血CD19+CD5+B细胞(B1)及CD19+CD5+CD1d+B细胞(B10)的比例,探讨黄芩苷对其体外表达的影响。方法:收集并分离活动期UC患者其外周血单核细胞(PBMC),以年龄性别相匹配的正常人PBMC为对照,经脂多糖(LPS)+黄芩苷体外刺激淋巴细胞,流式细胞术检测刺激前后外周血B1和B10细胞比例。结果:UC患者外周血中B1和B10细胞占CD19+B细胞的比例(4.83%±3.26%,0.97%±0.48%)低于正常对照组(16.16%±11.89%,5.99%±3.59%),P<0.05,LPS+黄芩苷体外刺激能上调UC患者B10淋巴细胞的比例(P=0.02),但对正常人该淋巴细胞亚群未见明显影响。结论:B1和B10细胞可能在UC病程中发挥免疫调节作用,黄芩苷可能对UC患者的B10细胞有上调作用。
Objective： The detection and analysis the proportion of CD19＋CD5＋B cells （B1） and CD19＋CD5＋CD1d＋B cells （B10） in peripheral blood of patients with ulcerative colitis （UC）, exploring the effect of baicalin on up-regulating both the two subpopulations expression in vitro. Method： Separate peripheral blood mononuclear cells （PBMC） of UC patients, using the PBMCs of age and gender-matched helthy people as normal control, lymphocytes were stimulated by lipopolysaccharide（LPS）＋baicalin in vitro, B1 and B10 cells proportion were detected by the flow cytometry before and after stimulation. Result： The proportion of B1 and B10 cells are decrease in UC patients peripheral blood compare to the normal control group（P=0.029,0.049）. In vitro, LPS＋baicalin stimulation raised the proportion of B10 lymphocytes of UC patients（P=0.02）, but no significant impact on normal subjects. Conclusion： UC may be related to the expression of B1 and B10 cells, baicalin might upregulates the proportion of B10 cells of UC patients.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第10期245-248,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省自然科学基金项目(10152402301000003)
