摘要
摸索建立大鼠慢性支气管炎（慢支）模型的新方法。用气管内注入脂多糖（ＬＰＳ）法建立大鼠慢支模型。观察光镜及电镜下气管、支气管及肺组织病理学改变。气管及支气管上皮脱落，杯状细胞增生，粘液腺增生肥大，气管支气管壁见慢性炎细胞浸润，管壁增厚，管腔充满粘液及大量以中性粒细胞为主的炎细胞，支气管平滑肌增生肥厚，肺气肿形成电镜下气管支气管纤毛柱状上皮细胞变性，数量减少、复合纤毛形成；ＢＡＬＦ白细胞总数及中性粒细胞数较对照组显著增多（Ｐ＜０．００１）。结论为气管内注入ＬＰＳ（２００ ｇ／２００ Ｌ）可制备大鼠慢支模型，气管支气管及肺组织病理学和ＢＡＬＦ细胞学改变符合人类该病的病理表现，可应用于实验研究。
The purpose of the study is to investigate a new method of establishment a rat chronic bronchitis model for experimental use. The animal model was produce by intratracheal instillation of small dose of lipopolysaccharide (LPS 200 g/ L). Pathologic changes were observed in tracheal, bronchi and lung tissues. Total and different white blood cell counts of BALF were carried out. (1) Significant reduction in weight gaining was seen in chronic bronchitis model group than that in control group (P<0.05). (2) Significant increase in numbers of goblet cells and mucous glands in tracheal and bronchi, loss of cilia of epithelial cells, infiltration of lymphocytes, monocytes and neutrophils in airway wall, accumulation of neutrophils and mucous in airway Lumens and pulmonary emphysema were observed under light microscope; degeneration of ciliated epithelium , compound cilias, numerous mucous granule in goblet cells, wide gap between epithelial cells and hyperplasia of type alveolar epithelial cells were seen under electron microscope; in chronic bronchitis model group Significant increases in total white blood cell numbers and neutrophils in BALF were found in rat chronic bronchitis group (p<0.001 ). A typical rat chronic bronchitis model could be produced by intratracheal instillation of LPS. The rat chronic bronchitis model shared many features of human chronic bronchitis in pathologic and BALF cytologic changes.
出处
《基础医学与临床》
CSCD
2000年第4期87-90,共4页
Basic and Clinical Medicine
