摘要
目的初步探讨Thl7细胞及其维甲酸受体相关孤儿受体(retinoid-ralated orphan receptorγt,RORγt)在肺纤维化中的作用。方法 64只雄SD大鼠随机分为对照组(N)、肺纤维化模型组(B组)、地塞米松治疗组(D)。采用气管内注入博来霉素致大鼠肺纤维化模型.苏木精-依红(HE)观察肺组织病理形态学变化并测肺组织中羟脯氨酸(HYP)含量;PCR检测肺部RORγtmRNA的表达;流式细胞术计数肺组织CD4+IL-17+Th17细胞;ELISA测血清IL-17水平.结果 (1)B、D组肺组织病理呈现逐渐由肺泡炎至纤维化动态改变过程,B、D组肺组织内HYP含量在不同时间点均高于N组(P<0.05),28 d时数值最高;(2)与N组比较,B、D组在不同时间点肺组织中TH17细胞和RORγtmRNA、血清中IL-17表达均明显增高(P<0.05),以造模7d最为显著。结论 TH17细胞、RORγtmRNA和血清IL-17表达增高在肺纤维化形成过程中起重要作用。
Objective To investigate the effect of Th17cell and retinoid-ralated orphan meceptor γt （RORγt） on rals with pulmo- nary fibrosis. Methods 64 SD rats were ramlomly divided into 3 groups of normal control group （ Group N） , model control group （ Group B） and dexmethasone treatment group （Group D）. Pulmonary fibrosis model was established by intratracheal injection of bleomycin. The normal control group received intra-tracheal instillation of saline instead. Lung tissues were restored to analyze the pathological changes and to determine the level of hydroxyproline in lung tissue. The levels of IL-17 in serum we~ measured by enzyme-linked immunosorbent assay （ELISA）. The expressions of ROR^t mRNA were measured by reverse transeription-polymerase chain reaction （RT-PCR）. The propor- tion of CD4 ＋ IL-17 ＋ Th17 cells was evaluateci by flow cytometry. Results （ 1 ） In the model group and dexamethasone treatment group, lung tissue biopsy showed the dynamic process of change from alveolitis to fibrosis gradually. The level of hydroxyproline （HYP） in lung tissue was higher in the group B and D than in the control group （ P 〈0.05 ） , which reached the highest on the 28th day. （2） The levels of CD4 ＋ IL-17 ＋ Thl7 cells and RORγt mRNA in lung tissue and IL-17 in serum were significantly higher in lhe group B and D than in the group N at all time points （P 〈0.05） , and the difference reached the largest on the 7th clay. Conclusion The high expression of TH17, RORγt mRNA and IL-17 suggests that it may involve in the formation of pulmonary fibrosis.
出处
《临床肺科杂志》
2013年第6期1075-1078,共4页
Journal of Clinical Pulmonary Medicine
基金
黑龙江省科学技术研究面上项目(12521528)
黑龙江省研究生创新科研项目(YJSCX2012-361HLJ)
