摘要
为建立灵敏、可靠的食品中鸭源性成分鉴定方法,确定了一对可特异地检测出牛肉中所掺杂鸭肉成分的引物,建立了SYBR Green Ⅰ实时PCR技术检测鸭肉的方法。结果表明:利用SYBR Green Ⅰ实时PCR技术可检测到鸭肉DNA,通过熔解曲线能有效地区分特异性产物,检测灵敏度可达到1pg DNA。同时利用该方法可检测出熟牛肉、鸭肉混合样品中的鸭肉成分。SYBR Green Ⅰ实时PCR方法能快速、特异、灵敏的检测鸭肉成分。
To establish a method which was use to detect the chosen to detect the duck in beef, fluorescent SYBR Green duck-derived components, one pair of primer was I Real-time PCR was developed for detection of the duck. The results showed that, duck gene DNA fragment could be detected and specially analyzed by melting curves, the detection limit was 1 pg DNA. Duck could be detected by the method from cooked duck and beef mixture. SYBR Green I Real-time PCR was the effective methods for detecting duck.
出处
《中国农学通报》
CSCD
2013年第11期16-19,共4页
Chinese Agricultural Science Bulletin
基金
湖南省科技重大专项"湖南省食品安全监控技术体系研究与示范"(2010FJ1009)
"十二五"国家科技支撑计划项目"旅游餐饮安全保障及基地原材料安全控制技术研究与示范"(2012BAK17B17)
长沙市科技计划重点项目(K1112002-31)
