摘要
目的:探讨舒郁胶囊对慢性应激诱导的经前期综合征(PMS)肝气郁证大鼠的治疗作用及谷氨酸(Glu)与γ-氨基丁酸(GABA)比值在其作用机制中的改变。方法:根据旷场实验及糖水偏好实验大鼠随机分为空白组、模型组、氟西汀组、舒郁胶囊组与柴胡皂苷组。药物治疗作用在给药4d后通过体质量、旷场实验及糖水偏好实验进行评价。微透析收集海马细胞外液,HPLC检测透析液中的Glu及GABA含量。结果:与空白组比较,模型组糖水偏好系数、旷场总得分及体质量显著下降(P<0.01,P<0.05),舒郁胶囊组与模型组相比该3指标均显著上升(P<0.05)。结论:舒郁胶囊可能通过抑制中枢神经系统海马中Glu与GABA比值下降治疗PMS肝气郁证。
Objective： To investigate the therapeutic effect of Shuyu Capsule （SYC） on premenstrual syndrome （PMS） depression and its efficacy mechanism on ratio of glutamate （Glu） to γ-aminobutyric acid （GABA） in PMS depression model of rats induced by chronic stress constraint. Methods： Rats were randomly divided into the control group, model group, fluoxetine group, SYC group and saikosaponins group according to the results of open field test （OFT） and sucrose preference test （SPT）, and the therapeutic effect of drugs was evaluated by body mass, OFT and SPT after four days of treatment. Hippocampal extracellular fluid was collected with microdialysis, and levels of Gin and GABA in microdialysate were measured by high performance liquid chromatography （HPLC）. Results： Compared to the control group, the sucrose preference coefficient, total score of OFT and body mass in the model group lowered sharply （P〈0.01, P〈0.05）, and the three indicators in the SYC group were significantly increased by preventive treatment with SYC compared to the model group （P〈0.05）. SYC exhibited effective inhibition on the decrease of Glu to GABA ratio in hippocampus of PMS depression rats. Conclusion： SYC has good activity in improving symptoms of PMS depression possibly by increasing of Glu to GABA ratio and regulating the disturbance of Glu and GABA levels in the central nervous system （CNS）.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第5期1219-1227,共9页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
National Natural Science Foundation of China(Key Program)(No.30930110),National Natural Science Foundation of China(No.30973688,No.81173151,No.81102537,No.81173163)~~
关键词
经前期综合征
舒郁胶囊
谷氨酸
γ-氨基丁酸
Premenstrual syndrome depression
Shuyu Capsule
Glutamate
γ-aminobutyric acid
