摘要
目的探讨血清TBX3、CA15-3及CEA联合检测在乳腺癌诊断中的临床价值。方法收集140例乳腺癌患者血清标本作为实验组,78例良性乳腺疾病患者血清标本作为良性对照组,100例健康女性血清标本作为正常对照组。血清TBX3含量采用ELISA法检测,血清CA15-3和CEA表达水平用化学发光法检测。所有数据采用SPSS 18.0软件进行统计分析。结果100例健康女性血清TBX3含量为1.72(1.12～2.49)ng/ml,按百分位数法(P95)确定其正常人群上限界值为5.50ng/ml。与良性对照组相比,乳腺癌组血清TBX3、CA15-3和CEA含量均显著升高,差异有统计学意义(P<0.05)。以临床分期为参照指标,TBX3、CA15-3及CEA在Ⅳ期乳腺癌患者血清中的表达水平明显高于其他各期患者(P<0.05)。血清TBX3、CA15-3和CEA对全组乳腺癌的敏感度分别为48.57%、36.43%和31.43%,特异性分别为91.03%、98.72%和100.00%,准确度分别为68.33%、58.72%和55.96%。血清TBX3、CA15-3和CEA联合检测对乳腺癌的敏感度为60.00%,特异性为89.74%,准确度为70.64%。在64例复发或转移性乳腺癌中,血清TBX3、CA15-3和CEA的敏感度分别为64.06%、68.75%、62.50%,而三者联合检测的敏感度则高达79.69%。联合检测与单项指标检测相比,诊断敏感度明显提高(P<0.05)。结论血清TBX3、CA15-3和CEA联合检测能提高乳腺癌的诊断敏感度,对乳腺癌的临床诊断、病期判断以及监测复发或转移方面均有重要价值。
Objective To investigate the clinical value of combined test of serum TBX3, CA15 - 3 and CEA levels in the diagnosis of mastocarcinoma. Methods Three hundred and eighteen females were enrolled in this study： one hundred were healthy females;one hundred and forty were histologically confirmed mastocarcinoma and seventy - eight cases were diagnosed with benign breast lesions. Serum level of TBX3 was measured by enzyme linked immunosorbent assay（ELISA） ,and serum levels of CA15 -3 and CEA were detected in the chemiluminescence method. Statistical analysis was performed using SPSS 18.0. Results The serum TBX3 level of healthy females was 1. 72 （ 1.12 - 2.49） ng/ml, and the cut - off value of serum TBX3 for healthy people was 5.50ng/ml. Elevated serum levels of TBX3, CA15 - 3 and CEA was significantly observed among patients with mastocarcinoma compared to benign females（P 〈 0.05 ）. According to clinical -pathologic stage,the levels of TBX3,CAI5 -3 and CEA in serum were statistically higher in stage IV patients than those in stage I - 11I （P 〈 0.05 ）. The sensitivity of serum TBX3, CA15 - 3 and CEA for diagnosis in breast carcinoma were 48. 57% , 36.43% and 31.43% ,and the specificity were 91.03% ,98.72% and 100.00% ,and the aceuraey rates were 68.33% ,58.72% and 55.96% ,respec- tively. In combined assay of serum TBX3, CA15 -3 and CEA for the total mastocarcinoma,the sensitivity was 60.00% ,the specificity was 89.74% ,the accuracy rate was 70.64%. Furthermore,in the 64 mastocareinoma patients with recurrence diseases or distant metastasis, the sensitivity of TBX3 ,CA15 -3 and CEA were 64.06% ,68.75% and 62.50% ,respectively,while the sensitivity of combined detection was up to 79.69%. Compared with the single assay, combined analysis had much higher diagnostic sensitivity （P 〈 0.05 ）. Conclusion The combined detection of serum TBX3, CA15 - 3 and CEA can increase the diagnostic sensitivity,and the serum marker analysis is shown to be significant for the identification of mastocarcinoma, clin
出处
《医学研究杂志》
2013年第4期118-121,共4页
Journal of Medical Research
基金
杭州市科技发展计划项目(20100633B05)
