摘要
目的:研究胃癌及癌旁组织中E-钙粘附素(E-cadherin)和环氧合酶(cyclooxygenase:COX)-2的表达,探讨蛋白表达与临床病理指标及与癌旁黏膜表达的关系。方法:采用组织芯片及免疫组织化学技术,对胃癌组织121例及118例和癌旁组织113例及111例。分别检测E-cadherin及COX-2,并分析二者蛋白表达情况与临床病理指标相关性。结果:胃癌E-cadherin阳性率为76.9%(93/121),其表达降低与肿瘤大体分型(P=0.014)、Lauren分型(P<0.001)、肿瘤分化(P=0.004)及pTNM分期(P=0.024)等显著相关,分化差分期晚的肿瘤E-cadherin表达要显著低于分化好分期早的肿瘤。癌旁黏膜组织E-cadherin均呈阳性表达,与肿瘤无显著相关。COX-2蛋白阳性率为38.1%(45/118),其过表达与淋巴结转移相关(P=0.048),有淋巴结转移的表达率高,与其它病理指标无明显相关性。结论:E-cadherin及COX-2分别从肿瘤分化、分期及淋巴结转移等方面预测胃癌患者预后,二者互补,联合应用可做为临床预后判断的参考指标。
Objective： This work aimed to evaluate the expressions of E-cadherin and cyclooxygen-ase-2 （COX-2） in primary gastric carcinoma and adjacent mucosa, and to investigate the correlation between abnormal expression of the said proteins and the clin- ico-pathological features of gastric adenocarcinoma. Methods： Immunohistochemical staining was used to determine the expression of E-cadherin and COX-2 on tissue microarray slices obtained from gastric carcinoma and adjacent mucosa of over 100 cases from the Cancer Institute and Hospital of Chinese Academy of Medical Science between February 2005 and October 2006. Results： The positive expressions of E-cadherin and COX-2 were 76.9% （93/121） and 38.1% （45/118）, respectively, in gastric carcinoma, whereas E-cadherin expression was 100% in 113 cases with adjacent mucosa. The positive expression of E-cadherin was significantly correlated with clini- cal characteristics such as gross type （P=-0.014）, differentiation （P=0.004）, and pTNM stage （P=-0.024）. COX-2 expression was higher in gastric cancer cases with lymph node me-tastasis than those without, and the result was significantly different （P=0.048）. Conclu- sion： E-cadherin is a tumor-inhibiting factor and COX-2 is a carcinogen. Positive expression of E-cadherin and negative expression of COX-2 are correlated with better prognosis. E-cadherin and COX-2 may thus be used as potential markers of tumor progression and prognosis.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第8期458-461,共4页
Chinese Journal of Clinical Oncology
