摘要
目的探讨珠海市冬春季节儿童诺如病毒（NV）、扎如病毒（sV）和星状病毒（AstV）等3种重要病毒性腹泻的流行病学特征。方法采集2009年11月21日至2010年4月3日在珠海市妇幼保健院就诊的腹泻儿童粪便样本，选取轮状病毒和腺病毒筛查阴性的样本，采用逆转录-聚合酶链反应（RT．PCR）技术检测NV、SV和AstV的特异基因片段，并对诺如病毒检测阳性的样本进行分子分型。结果3种病毒季节总检出率为21．49％，其中2009年12月的检出率最高，为29．05％，而2010年2月检出率最低，为12．20％，87．96％的阳性样本来自0—30月龄患儿。NV、SV及AstV的季节检出率分别为14．70％、2．75％和4．04％，3种病毒中以NV和SV各月份阳性检出率差别较大，而AstV较为一致，12—18月龄患儿NV检出率最高（34．09％），60～120月龄患儿sV检出率最高（12．5％），24～30月龄患儿AstV检出率最高（16．67％）。诺如病毒经分子分型后均为GII型。结论NV是引起2009年冬春季节珠海市儿童病毒性腹泻的主要病原之一，SV与AstV也是重要的病原，应加强对婴幼儿NV、SV和AstV等3种重要病毒性腹泻的监测和防控工作。
Objective To explore the epidemiological characteristics of viral diarrhea of norovirus （NV） , sapovirus（SV） and astrovirus（AstV） among children in Zhuhai during winter and spring. Methods Stool specimens were collected from children with viral diarrhea in Maternal and Child Health Hospital of Zhuhai from November 21, 2009 to April 3, 2010. Nucleic acid of NV, SV and AstV from negative specimens of rotavirus and adenovirus were detected by using Reverse transcription-polymerase chain reaction （RT-PCR） , and the types of positive samples of NV were also classified at the same time. Results The total detection rate of the three viruses is 21.49 percent, the highest detection rate is 29.05% in December 2009, the lowest detection rate is 12. 20% in February 2010, 87.96% of positive specimens were from children patients aged from 0 to 30 months. The season detection rate of NV, SV and AstV are 14. 70% , 2.75% and 4.04% respectively. There were significant differences of NV and SV detection rates in every month of the season, whereas the AstV detection rate was comparatively stable. The highest detection rate of NV is 34. 09% in children patients aged from 12 to 18 months, the highest SV detection rate is 12. 5% in children patients aged from 60 to 120 months, and the highest AstV detection rate is 16. 67% in children patients aged from 24 to 30 months. All the NV were belong to G II genogroup. Conclusions NV is one of the main pathogens causing viral diarrhea among children in Zhuhai during winter and spring, SV and AstV are also important pathogens. So we should strengthen the monitoring of viral diarrhea caused by NV, SV and AstV in infants and young children.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2013年第2期98-101,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家传染病防治科技重大专项课题传染病监测技术平台项目（2009ZX10602-02）
珠海市科技计划项目（PC20081002）
