摘要
目的 探讨新的痰标本收集和保存方法用于肺癌的辅助诊断。方法 分别用常规法和改良Saccomanno法 (以下简称改良S法 ) ,对 12 5例经细胞或组织病理学确诊的肺癌患者的痰标本进行处理。部分标本尚进行了p5 3突变基因检测和流式细胞术测定p5 3和p2 1蛋白。结果 用改良S法获得的痰标本涂片示癌细胞形态完整 ,背景杂质少 ,更易辨认。 12 5例患者常规法涂片发现肺癌细胞阳性者3 5例 ,改良S法发现 69例 ,阳性率分别为 2 8%和 5 5 .2 % ,二者间有显著性差异 (P <0 .0 1)。常规法和改良S法检出的阳性患者中分别有 2 6例和 5 8例能明确病理类型 ,二者比较差异也有显著性 (P <0 .0 1)。改良S法阳性率与肺癌病理类型、TNM分期和肺癌类型有密切关系 (P均 <0 .0 5 )。改良S法处理的痰标本中 ,41例用PCR SSCP 银染法检测p5 3基因突变 ,阳性率 5 3 .7% ( 2 2 / 41) ;3 0例用流式细胞术测定p5 3和p2 1蛋白含量 ,阳性率分别为 40 % ( 12 / 3 0 )和 5 3 .5 % ( 16/ 3 0 )。结论 改良S法处理的痰标本能很好地保存肺癌细胞形态和成分 ,易于检出。标本也适于肺癌相关的基因及蛋白检测。
Objective To compare the improved Saccomanno method with conventional method not only on concentrating and detecting cytological sputum but also on preserving sputum samples for further molecular biological and flow cytometry (FCM) investigation in lung cancer patients. Methods A total of 125 cases were identified as lung cancer by histological or cellular pathology, and sputum samples from these patients were collected by conventional method and improved Saccomanno method respectively for sputum smear. Then, some sputum samples collected by improved Saccomanno method were studied by FCM and/or PCR SSCP silver stain. Results The positive smear rates were 55.2%(69/125) by improved Saccomanno method and 28%(35/125) by conventional method respectively (P<0.01). Of the 125 cases, 58 by improved Saccomanno method and 26 by conventional method were confirmed for histological types of lung cancer respecitvely, and a highly significant difference was observed between the two groups (P< 0.01 ). The positive smear rate by improved Saccomanno method had significant correlation with the clinical characteristics such as histology, TNM stage, and type of lung cancer. Moreover, of the samples collected by improved Saccomanno method, 41 cases were selected to detect p53 point mutation by PCR SSCP silver stain and 30 cases were selected to quantitate p53 and p21 protein by FCM technique successfully, the positive rates were 53.7%(22/41), 40%(12/30) and 53.3% (16/30) respectively. Conclusion The results indicate that improved Saccomanno method has a higher value than conventional method not only on concentrating and detecting cytological sputum but also on preserving sputum sample for further molecular biological study.
出处
《中国肺癌杂志》
CAS
2000年第4期280-283,共4页
Chinese Journal of Lung Cancer
基金
卫生部科学研究基金!(96 2 0 1 5)&&
