摘要
大豆蛋白的体外酶解物——大豆肽具有与钙结合形成复合物并促进肠道细胞(Caco-2)钙吸收的能力。大豆蛋白主要由大豆球蛋白(Glycinin,11S)和β-伴大豆球蛋白(β-conglycinin,7S)组成,两者占大豆中蛋白质组成的70%～80%。本文采用平衡透析法比较7S和11S酶解物的钙结合量,并以体外Caco-2单层细胞模型评价7S大豆肽-钙复合物和11S大豆肽-钙复合物对肠道细胞钙吸收的影响。试验结果表明,11S酶解物的钙结合量[(159.90±9.40)mg/g]高于7S酶解物[(140.73±6.88)mg/g](P<0.05)。11S大豆肽-钙复合物肠道细胞的钙转运量为23.72μg/孔,显著高于7S大豆肽-钙复合物(17.96μg/孔)和对照(17.46μg/孔)(P<0.05)。本研究表明,与7S大豆肽-钙复合物相比,11S大豆肽-钙复合物在大豆-钙复合物促进肠道细胞钙吸收和转运过程中起重要的作用。
Abstract Soybean protein hydrolysates（SPHs） can bind with calcium and promote calcium uptake by Caco-2 cells, while the effect of β-conglycinin （7S） and glycinin （llS）, two major fractions in soybean protein, is still not known. In this study, Caeo-2 monolayer cells were used to evaluate calcium transport caused by 7S and llS hydrolysates. The re- sult showed that the transported calcium by Caco-2 cells was 23.72 μg/well in the presence of 5 mg/mL llS hy- drolysates, which is significantly higher than 7S hydrolysates and control （17.96 μg/well and 17.46μg/well, respectively） （P〈0.05）. This result indicated that llS hydrolysates play more important role in SPHs promoting calcium uptake than 7S hydrolysates.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2013年第4期185-190,共6页
Journal of Chinese Institute Of Food Science and Technology
