摘要
目的 :探讨抑癌基因p16和Rb在喉肿瘤中的表达及其相互关系。方法 :用免疫组织化学方法观察 4 0例喉鳞状细胞癌p16和Rb基因的表达。结果 :喉癌标本中p16和Rb阳性表达率分别为 4 2 5%和57 5% ,其中 7例肿瘤组织 ( 17 5% )p16和Rb基因均阳性表达 ,未见同一肿瘤组织中两者均缺失。在喉癌的临床类型、分期及组织分化程度方面p16和Rb基因的阳性表达率差异有显著性 (P <0 0 5)。结论 :p16和Rb在喉肿瘤的发生发展过程中起重要作用 ;但两者同时表达缺失较少见。
objective:To study the expression and correlation of p16 with retinoblastoma genes in laryngeal carcinoma.Method:The correlation and expressions of p16 and Rb were investigated by the immunohistochemical method in 40 specimens with laryngeal squamous cancer.Results:The positive expression rates of p16 and Rb were 42.5%(17/40) and 57.5%(23/40) respectively.Both p16 and Rb genes could be detected in 17.5% of all carcinomas,but lacking both of the two genes was not found in the same tissue.There was a significant difference of the positive expressions of p16 and Rb genes in laryngeal carcinoma through different clinical types,stages and histopathological grades (P<0 05) Conclusion:The expressions of p16 and Rb genes play an important role in the genesis and progressive process of laryngeal carcinoma.But loss of both tumor suppressors appears to be distinctly uncommon.
关键词
喉肿瘤
免疫组化
P16基因
视网膜母细胞瘤
Laryngeal neoplasmas
Immunohistochemistry
Genes,p16
Genes,retinoblastoma
