摘要
自然环境中有超过99%的微生物是不可培养的。使用宏基因组技术,人们首次实现了对不可培养微生物的开发,这为探索和挖掘新的酶制剂和活性小分子提供了更为有力的技术手段。本文重点介绍宏基因组技术最新的研究进展及其在新酶开发中的成功应用,以期利用宏基因组技术推进新酶开发,为其在食品工业中的应用提供有效途径。
More than 99% of microbes in natural environment are uncultivable.These uncultivable microorganisms have been exploited for the first time by using metagenomic technology,especially as a more powerful technique for the exploration and mining of novel enzymes and active small molecules.In this paper,the current research advances of metagenomic technology and successful applications in the development of new enzymes have been summarized,which will accelerate the development of new enzymes through metagenomic technology and provide an effective strategy for its application in the food industry.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第9期404-407,共4页
Food Science
关键词
宏基因组
宏基因文库
酶制剂
富集技术
DNA提取
metagenomic technology
macro-gene library
enzyme
enrichment technology
DNA extraction