摘要
本文综述鱼类肌原纤维结合型丝氨酸蛋白酶(myofibril-bound serine proteinase,MBSP)的分离纯化、酶学特性、分子生物学及基因重组表达等方面的研究进展。鱼类MBSP是一种弱碱性蛋白酶,它普遍存在于鱼类肌肉中。MBSP降解肌原纤维蛋白是造成鱼糜凝胶劣化的主要原因。同时,由于鱼类MBSP具有良好的热稳定性及对精氨酸或赖氨酸残基羧基端特异分解的底物特异性,因此,该蛋白酶有望开发成为一种新颖的工具酶应用于蛋白质质谱鉴定等研究领域。
This paper reviews the recent progress in the purification,characterization,molecular cloning and recombinant expression of myofibril-bound serine proteinases（MBSP） from different species of fish.As a weakly alkaline proteinase,MBSP is extensively found in fish skeletal muscles.The degradation of myofibrillar proteins by MBSP is mainly resposible for the modori phenomenon during fish surimi production.From this reviwe,we conclude that fish-derived MBSP hold great promise as a novel tool in mass spectrometric identification of proteins due to their excellent stablity to heat and substrate specificity to decompose the carboxyl chains of arginine or lysine residues.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第9期336-339,共4页
Food Science
基金
国家自然科学基金面上项目(30571450
20872049)
