摘要
从濒危药用植物七叶一枝花的叶片中分离得到内生真菌Penicillium sp.(NO.4),活性筛选结果显示Penicillium sp.(NO.4)显示出较强的抑制肝癌细胞活性,通过固体发酵,乙酸乙酯萃取得浸膏,其浸膏经硅胶柱层析,Sephadex LH-20,半制备型HPLC等分离手段分离得到八个化合物,经核磁共振,质谱等手段鉴定其结构分别为1,3,14-三甲氧基-6-甲基-9,10-蒽醌(1),bostrycin(2),isorhodoptilometrin(3),physcioin(4),emodin(5),aloesol(6),coniochaetone B(7),2,5-dimethyl-7-hydroxychromone(8),其中化合物1为一新天然产物。体外细胞毒活性显示化合物1,3,14-三甲氧基-6-甲基-9,10-蒽醌(1),bostrycin(2)和isorhodoptilometrin(3)对肝癌细胞有较强的抑制活性,其IC50分别为15.6,6.5,13.2μg/mL,化合物4～8则没有显示生物活性。
During the investigation of bioactive metabolites from the endangered plants in Shennongjia District, an extract from an endophytic fungus ,PeniciUiumsp. displayed strong inhibition activity to Hep G2 cells ,which led to the isolation of eight polyketides,including a new natural product 1,3,14-trimethoxyl-6-methyl-9,10- anthraquinone （ 1 ） and seven known compounds bostrycin （2）,isorhedoptilometfin （3）, physcioin （4）, emodin （ 5）, aloesol （ 6）, eoniochaetone B （7） and 2,5-dimethyl-7-hydroxyehromone （8）. And the eight compounds were evaluated to Hep G2 cell lines ,the re- sults showed that the compound 1-3 inhibited the Hep G2 cell lines with ICs0 15.6,6.5,13.2 p^mL, but compounds 4- 8 displayed no significant inhibition activity to Hep G 2 cell lines in vivo.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第4期431-434,493,共5页
Natural Product Research and Development
基金
国家自然科学基金项目(21002058,21272137)
关键词
内生真菌
七叶一枝花
聚酮类化合物
抗肝癌活性
次级代谢产物
endophytic fungi
par/s polyphylla Sm.
polyketides
anti-hepatocarcinoma activity
secondary metabolites.
