摘要
以PCR产物克隆后测序的方法测定了贵州赤水桫椤国家级自然保护区3个种群59个个体的叶绿体DNA（cpDNA）atpB—rbcL非编码区序列。序列长度介于727～732bp之间，具长度多态性，A＋T百分比含量较高，介于63．27％～63．89％之间。遗传多样性水平较低，表现出较高的单倍型多样（h=0．639）和低的核苷酸多样性（Dij=0．00028）并存的特征，提示现有种群可能源自一个有效群体规模较小的种群的快速扩张，扩张时间尚短，不足以形成更为复杂的遗传结构。种群间的高基因流Nm、低遗传分化度峙、AMOVA分析以及DNA歧异度结果一致显示各种群间无明显的遗传分化。
Chloroplast DNA （cpDNA） atpB-rbcL noncoding sequence of Alsophila spodophylla, collected from three populations distributed at Asphole National Nature Reserve in Chishui of Guizhou province were sequenced. Sequence length varied from 726-732 bp, showing length polymorphism, with high A＋T content between 63.27% and 63.89%. High level ofhaplotype diversity （h=0.639） and a low nucleotide diversity （Dij=0.000 28） were detected in the populations of A lsophila spinulosa. This suggested rapid demographic expansion from a small effective population, and, since then, there has been insufficient time to form a more complicated population structure. Observed high gene flow Nm, low FST, AMOVA analysis and DNA divergence data consistently indicated that no genetic differentiation should occur at the population level.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2013年第2期212-215,共4页
Genomics and Applied Biology
基金
国家自然科学基金资助项目(30771763
30170101)资助
