摘要
目的了解HIV感染者或AIDS患者AIDS相关支原体生殖支原体(Mycoplasma genitalium,Mg)、发酵支原体(Mycoplas-ma fermentans,Mf)、穿通支原体(Mycoplasma penetrans,Mpe)和梨支原体(Mycoplasma pirum,Mpi)的感染状况并分析其与疾病发展的关系,为进一步研究AIDS相关支原体在HIV感染者/AIDS患者免疫功能变化中的作用提供依据。方法对江苏省312名男性HIV感染者/AIDS患者采用流行病学横断面调查的方法,在调查对象知情同意的基础上开展问卷调查,同时采集首段尿、静脉血标本。用PCR法检测支原体,流式细胞仪检测外周血CD4+T淋巴细胞,ELISA双抗体夹心法检测细胞因子水平。结果江苏省男性HIV感染者/AIDS患者中,除无Mpe感染外,存在其他3种AIDS相关支原体Mg、Mf、Mpi的感染,检出率分别为26.9%、13.8%和21.8%。Mf的感染率在不同CD4+T细胞水平者间的差异有统计学意义(χ2=11.775,P=0.003)。对男性HIV感染者/AIDS合并AIDS相关支原体感染者血清细胞因子水平进行分析,合并支原体感染组的IL-4水平(4.437pg/mL)高于单纯HIV感染组(1.922 pg/mL),IL-6水平(0.431 pg/mL)低于单纯HIV感染组(0.532 pg/mL),差异均有统计学意义(P<0.001)。多因素logistic回归分析结果显示,苏南地区较苏北地区AIDS相关支原体感染危险性高(OR=2.388,95%CI:1.212～4.706),而苏中较苏南地区AIDS相关支原体感染危险性低(OR=0.447,95%CI:0.253～0.790);HIV感染者/AIDS患者积极进行抗逆转录病毒治疗者发生AIDS相关支原体感染的危险性较小(OR=0.545,95%CI:0.311～0.955)。结论江苏省男性HIV感染者/AIDS患者存在较高AIDS相关支原体的感染;支原体感染与机体免疫状况具有一定的相关性,但其关联机制还需要进一步深入探讨。
Objective To investigate the prevalence of infection with AIDS-related mycoplasma,including Mycoplasma genitalium（Mg）,Mycoplasma fermentans（Mf）,Mycoplasma penetrans（Mpe） and Mycoplasma pirum（Mpi）,in male HIV carriers and AIDS patients in Jiangsu province,and analyze the correlation between the infection of mycoplasma and the development of AIDS to provide the evidences for the role of AIDS-related mycoplasma in the alterations of immune function in latent HIV carriers and AIDS patients.Methods A cross-sectional epidemiological questionnaire survey was performed for 312 male HIV carriers and AIDS patiets following informed consent.The first void urine and venous blood samples from the males included in this study were collected.Mycoplasma was detected with PCR,CD4＋ T lymphocyte was measured with flow cytometry and the level of cytokines was determined by ELISA.Results Among the studied males,3 species of AIDS-related mycoplasma were detectable except Mpe with infection rates of 26.9%,13.8% and 21.8% for Mg,Mf and Mpi respectively.There were significant differences of the infection rates of Mf among different CD4＋ T-cell levels（χ2=11.775,P=0.003）.The level of IL-4（4.437 pg/mL） in the males who were infected by both of HIV and mycoplasma was significantly higher than that in the males with single HIV infection（1.922 pg/mL）,but the level of IL-6（0.431 pg/mL） in the males with complicated infection was lower than that of the single HIV infection（0.532 pg/mL）（P＆lt;0.001）.Multiple-factor logistic regression analysis showed that the risk of AIDS-related mycoplasma infection in south Jiangsu was higher than that in north Jiangsu（OR=2.388,95%CI： 1.212-4.706） and middle Jiangsu（OR=0.447,95%CI： 0.253-0.790）.The risk of AIDS-related mycoplasma infection in the males undergoing active antiretroviral treatment was the lower than that without ART（OR=0.545,95%CI： 0.311-0.955）.Conclusion It was noted that high pathogenic mycoplasma infection was found in the males wi
出处
《临床检验杂志》
CAS
CSCD
北大核心
2013年第3期210-214,共5页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(30872156)
