摘要
采用MEGA5.0软件对9个森林草莓无机磷转运蛋白序列及58个已知的其它植物磷转运蛋白的氨基酸序列进行聚类分析,发现5个基因与菌根磷转运蛋白基因分支聚在一起,初步判断是候选的草莓菌根磷转运蛋白。根据其基因序列设计引物获得了5个‘红颜’草莓候选菌根磷转运蛋白基因全长。实时荧光定量PCR结果表明,FaPT4、FaPT5和FaPT8在草莓菌根中显著上调表达,结合聚类分析结果,证明这3个基因是‘红颜’草莓菌根磷转运蛋白基因。
Phylogenetic tree was clustered by MEGA5.0 based on amino acid sequences of nine Fragaria vesca inorganic phosphate transporters and 58 known phosphate transporters of other plants. The results showed five inorganic phosphate transporters in Fragaria vesca were gathered the branch of mycorrhizal phosphate transporter, so these five genes were preliminarily predicted candidates of mycorrhizal phosphate transporter. Based on homologous sequences, full sequences of these five genes in Fragaria x ananassa Duch. ＇Benihoppe＇ were obtained. Real-time quantitative PCR showed that, FaPT4, FaPT5 and FaPT8 genes were dramaticly induced in the mycorrhizal roots of strawberry combined with cluster result above. Therefore, FaPT4, FaPT5 and FaPT8 were proven to be mycorrhizal phosphate transporter genes in Fragaria × ananassa Duch. ＇ Benihoppe＇ .
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第4期641-650,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31201610)
北京市属高等学校人才强教计划项目(PHR201108274)
北京市自然科学基金项目(6132005)
北京市科技新星项目(Z111105054511048)
关键词
草莓
菌根磷转运蛋白基因
基因克隆
聚类树
荧光定量表达分析
Fragaria × ananassa
mycorrhizal phosphate transporter gene
gene cloning
phylogenetic tree
quantitative expression
