摘要
目的探讨葛根素对肾癌GRC-1细胞多药耐药的逆转作用。方法体外培养GRC-1人肾细胞癌株,并分为空白对照组、阿霉素处理组(10mg·L-1ADM)、葛根素处理的对照组(0.48g·L-1Pur)、阿霉素联合葛根素处理组(10mg·L-1ADM+0.24g·L-1Pur或10mg·L-1ADM+0.48g·L-1Pur),药物处理24h后,采用细胞毒性实验检测GRC-1细胞的生存率,用流式细胞术(Annexin-V/PI双标记)及TUNEL法检测细胞的凋亡情况。结果以对照组细胞生存率为100%,ADM组细胞生存率为(69.7±0.04)%,显著低于对照组(P<0.05),经0.12、0.24和0.48g·L-1Pur联合用药后,细胞生存率分别为(70.1±0.03)%、(63.2±0.02)%和(42.1±0.04)%,均明显低于空白对照组(P<0.05),且0.24和0.48g·L-1Pur联合用药的细胞生存率显著低于ADM处理组(P<0.05);0.12和0.24g·L-1Pur单独用药的细胞生存率分别为(97.4±0.02)%和(90.1±0.01)%,与对照组比较差异无统计学意义(P>0.05),而0.48g·L-1Pur单独用药的细胞生存率为(75.0±0.03)%,显著低于空白对照组(P<0.05)。此外,与空白对照组相比,0.24和0.48g·L-1Pur处理细胞后,细胞凋亡率明显升高(P<0.05),且随Pur剂量增加而升高;而0.24和0.48g·L-1Pur联合用药组细胞凋亡率均明显高于ADM组(P<0.05)。结论葛根素可有效促进肾癌GRC-1细胞的凋亡,并可减轻GRC-1细胞对阿霉素的耐药性。
Objective To investigate the reversal effect of puerarin on mediated multidrug resistance in renal cell carcinoma line GRC-1. Methods GRC-1 cells were cultivated in vitro, and four groups were designed in this experiment： control group, adriamytin treatment group with 10 mg.L^-1 ADM, puerarin control group with 0.48 g.L ^-1 Pur, combined treatment group in which 10 mg.L^-1 AI）M±0 24 g.L^-1 Pur or 10 mg.L^- 1 ADM±0.48 g.L^-1 Pur respectively. After treatment with drugs for 24 h,MTT assay was employed to measure cell viability. Flow cytometry with Annexin-V/Pl double-labeled） and TUNEL method were used for detecting cell apoptosis. Results Compared with the control group in which the GKC-1 cell viability was 100%, cell viability in ADM group significantly decreased to （69.7 ± 0.04）% （P 〈 0.05）. In the conlbined treatment group, in which 10 mg.L^-1 ADM, combined with 0.12,0.24 and 0.48 g.L Pur was administered to GRC- 1cells, the cell viabilities were （70.1 ± 0.03）%, （63.2 ± 0.02）% and （42.1 ± 0.04）% respectively, which were obviously lower than that in the control group （P〈0.05）, The cells viabilities of two subgroups, in which 10 mg.L^-1 ADM was combined with 0.24 or 0.48 g.L^-1 Put, were much lower than that in the ADM group（P〈0.05）. The cell viabihties of the only 0.12 g·L^-1 or 0.24 g.L^-1 Pur treated group were respectively （97.4±0.02）% and （90.1 ±0.01）%, having no significant difference from the control group （P〉0.05）. But after treated by 0.48 g·L^-1 Put alone, the cell viability was （75.0 ± 0.03）%, much lower than that in the control group （P〈 0.05）. Compared with the control group, the apoptotic rates of GRC-1 cells in 0.24 and 0.48 g·L^-1 puerarin groups significantly increased in a dose-dependent manner （P〈0.05）. The apoptotic rates of 10 mg.L^-1 adriamycin plus 0.24 g.L^-1 puerafin or 0.48 g.L^-1 puerann groups were remarkablely higher than that of the ADM group （P〈 0.05）. Conclusion Puerarin may effectively promote
出处
《肿瘤药学》
CAS
2013年第2期96-99,共4页
Anti-Tumor Pharmacy
基金
山东省高校科技计划基金资助(J11LF90)
