摘要
目的:建立测定烟酸片中烟酸含量的方法。方法:采用高效液相色谱法。色谱柱为AglientC18,流动相为0.02mol/L磷酸二氢钾(pH6.8)-乙腈(90:10,V/V),流速为1.0ml/min,柱温为28℃,进样量为20μl,检测波长为261nm。结果:烟酸检测质量浓度在5.004～100.08μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均加样回收率为99.2%,RSD=0.9%(n=9)。结论:该法简便、准确、重复性好,适用于烟酸片的质量控制。
OBJECTIVE： To establish the method for the content determination of nicotinic acid in Nicotinic acid tablets. METHODS ： HPLC method was adopted. The separation was performed on Agilent-C18 column with mobile phase consisted of 0.02 mol/L potassium dihydrogen phosphate （pH 6.8）-acetonitrile （90： 10, V/V） at the flow rate of 1.0 ml/min. The column temperature was 28℃, and the injection volume was 20 p.1. The detection wavelength was set at 261 nm. RESULTS： The linear range of nico-tinic acid was 5.004-100.08μg/ml （r=0.999 8） with an average recovery of 99.2% （RSD=0.9%, n=9）. CONCLUSION： The method is simple, accurate and reproducible. It can be used for the quality control of Nicotinic acid tablets.
出处
《中国药房》
CAS
CSCD
2013年第16期1515-1516,共2页
China Pharmacy
关键词
烟酸片
烟酸
高效液相色谱法
含量测定
质量控制
Nicotinic acid tablets
Nicotinic acid
HPLC
Content determination
Quality control
