摘要
目的:对密银花的不同栽培变种进行分类研究,明确不同栽培变种密银花的遗传关系。方法:实地观察密银花的生物学特性,走访药农,确定密银花的变种;采用RAPD分子标记法分析不同栽培变种密银花的遗传关系。结果:对密银花的种质资源进行了调查、收集和整理,明确了密银花的栽培变种,建立了密银花栽培变种的检索表。同时,建立了密银花RAPD分析的扩增体系,共筛选出10条引物,得到扩增条带60条,其中多态性条带50条,多态率达到83.33%。结论:确定了密银花的栽培变种及其遗传关系,为保护密银花资源、合理开发利用及其药材质量的综合评价提供了科学依据。
Objective：To study the classification and genetic relationship among difference cultivated varieties of Lonicera japonica cultivated in Xinmi.Methods：Observed the biological characteristics of Lonicera japonica cultivated in Xinmi and visited farmers;Genetic diversity was analyzed by RAPD molecular biology techniques.Results：Classified the cultivated varieties and set up an index key.RAPD fingerprint was established,10 primers were chosen.A total of 60 DNA fragments,among which 50 bands was polymorphic,accounting for 83.33% which indicated the genetic diversity of the samples was good.Conclusion：Genetic relationship among different cultivated varieties are determined which provide scientific basis for resources protection,development of Lonicera japonica cultivated in Xinmi and its quality evaluation.
出处
《中药材》
CAS
CSCD
北大核心
2013年第1期6-9,共4页
Journal of Chinese Medicinal Materials
基金
2011年河南省科技创新人才计划(114200510014)
关键词
密银花
栽培变种
RAPD
Lonicera japonica Thunb.cultivated in Xinmi
Cultivated varieties
RAPD
