摘要
目的探讨骨髓间充质干细胞（MSC）对重症急性胰腺炎（SAP）胰腺损伤的修复作用。方法将60只大鼠随机（随机数字法）分为健康对照组、SAP模型组、Msc移植组、MSc加粒细胞集落刺激因子（G—CSF）联合组。免疫荧光检测MSC的转化率，生化法检测血清淀粉酶含量．ELISA法检测血清白介素IL-6质量浓度。结果骨髓间充质干细胞转化率上升，血清淀粉酶含量和白介素1L-6质量浓度下降，增加粒细胞集落刺激因子（G-CSF）联合治疗，MSc细胞量较少时，产生协同作用增强MSC的修复作用，MSC细胞量较大时，产生拮抗作用加重胰腺组织的损伤。差异有统计学意义（P〈0．05）。结论MSC对SAP有明显修复损伤的作用，并随MCS细胞量增多，修复作用增强。增加粒细胞集落刺激因子（G—CSF）联合治疗，根据MSC细胞量的不同，产生协同作用增强MSC的修复作用．或者产生拮抗作用加重胰腺组织的损伤。
Objective To investigate effects of bone marrow messenchymal stem calls （MSC） on severe acute panereatitis （SAP） in rats. Methods A total of 60 rats were randomly divided into normal group （n = 10）, the model of SAP group （n = 10）, the transplantation of the MSC group （n =20）, the combination of the MSC and the granulocyte colony-stimulating factor （G-CSF） group （n = 20）. The eonve^ion rate of MSC was detected by using immunoflnorescence methods, and the level of amylase in serum was assayed by using biochemical methods. Simultaneously, the level of interleukin-6 （ IL-6 ） in serum was determined by using enzyme linked immunosorbent assay （EL1SA）. Results The conversion rates of the MSC increased, and consequently, the levels of amylases and interleukin-6 in serum were reduced （ P 〈 0. 05 ）. When a small amount of the G-CSF was added to MSC, the therapeutic effects of the two different kinds of cells were synergistically strengthened, in the contrary, when a large numl^r of the G- CSF was added to MSC, the antagonism resulted between these two different kinds of cells gives ri~ to harmful effects on SAP. Conrlnsions The bone marrow mesenehymal stem cells have therapeutic effects on SAP. When the number of the bone marrow mesenchymal stem cells increases, the protective effects are enhanced.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2013年第4期395-399,共5页
Chinese Journal of Emergency Medicine
关键词
骨髓间充质干细胞
重症急性胰腺炎
保护作用
粒细胞集落刺激因子
Mesenchymal stem cells
Severe acute pancreatitis
Protective effects
Granulocytecolony-stimulating factor
