摘要
目的探索TET2基因（rs7679673，A）、LMTK2基因（rs6465657，T）和FAM84B基因（rsl2543663，C）的常见变异类型与前列腺癌患病风险的关系，分析前列腺癌患者的基因型与临床特征和生活习惯的关系。方法采用病例对照研究设计，比较病例组和对照组TET2基因（rs7679673，A）、LMTK2基因（rs6465657，T）和FAM84B基因（rsl2543663，C）的等位基因和基因型频率的差异，采用r检验分析各基因型与患者的确诊年龄、体质指数、Gleason评分、血清前列腺特异性抗原浓度、肿瘤分期等的关系，采用多因子降维（MDR）方法分析基因一基因交互作用。结果FAM84B基因（rsl2543663，C）的CC＋CA基因型在病例组和对照组中的频率分布差异有统计学意义（P=0．046）。TET2基因（rs7679673，A）和LMTK2基因（rs6465657，T）的AA、AC和CC基因型和等位基因在病例组和对照组中的频率分布差异均无统计学意义（均P〉0．05）。FAM84B基因（rsl2543663，C）的CC＋AC基因型在不同肿瘤分期前列腺癌患者中的分布频率，差异有统计学意义（P=0．002）。TET2基因的AA、AC和CC基因型在不同吸烟和饮酒情况的前列腺癌患者中的分布频率，差异均有统计学意义（均P〈0．05）。LMTK2（rs6465657，T）基因的TT＋TC和CC基因型在是否接受手术治疗的前列腺癌患者中的分布频率，差异有统计学意义（P=0．003）。树状图分析结果显示，TET2（rs7679673，A）和LMTK2（rs6465657，T）基因位点之间距离更近，存在更强的基因-基因交互作用。MDR方法验证最佳模型的检验平衡精度为0．5120，交叉验证一致性为100％（P=0．0270）。结论FAM84B基因可能与中国人前列腺癌患病风险和肿瘤分期有关，TET2、LMTK2和FAM84B基因的协同作用可能与前列腺癌的患病风险有关。
Objective To explore the association between the common variations of TET2 （ rs7679673, A） , MTK2 （ rs6465657, T） and FAM84B （ rs12543663, C ） genes and prostate cancer （Pea） risk in Chinese population in Beijing, and to understand the relationship between genotypes and phenotypes including clinical characteristics and life style, etc. in patients with prostate cancer. Methods Based on a case-control study, 124 patients with prostate cancer and 138 age-matched control subjects were recruited. Information of clinical phenotype and life style, etc. in the prostate cancer patients was collected. We compared the differences of allele and genotype frequencies of TET2 （ rs7679673, A）, LMTK2 （ rs6465657, T） and FAM84B （rs12543663, C） gene expressions between the two groups for the allele and genotype frequencies, and explored the relationship between different genotypes and clinical features such as patient age, BMI, Gleason score, PSA level and tumor stage, by Chi-square test in patients with PCa. Muhifac：ordimensionality reduction was used to detect the gene-gene interactions. Results The FAM84B （rs12543663, C） C carriers frequency had significant difference between the case group and the control group （X2 = 3. 980 P = 0.046 ; OR = 1. 883 ; 95% CI = 1. 006-3. 526）. The allele and genotype frequencies of TET2 gene （rs7679673, A） and LMTK2 gene （rs6465657, T） were not significantly different between the case group and the control group （P 〉 0.05 ）. Analysis of the genotypes and clinical phenotypes showed that the genetic type of FAM84B C carriers [ CX （ CC ＋ CA） ] were significantly associated with cancer stage （X2 = 9. 585; P = 0. 002; OR = 3. 740; 95% CI = 1. 580-8. 853）. Association between three loci and 12 kind of relevant outcomes was found in TET2 A carriers and the smoking and drinking patients （ all P 〈 O. 05 ）. Significant correlation was also found between LMTK2 （rs6465657, T） TX carriers and surgery （X2 = 8. 612; P = 0. 003; OR =0. 174
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2013年第4期262-267,共6页
Chinese Journal of Oncology
基金
基金项目：国家自然科学基金（30972709、81061120527、81241082）
北京医院重大基金（BJ-2010-30）
卫生部部属医院临床学科重点项目（10120101）
