摘要
通过添加外源聚γ-谷氨酸(γ-PGA),利用气相色谱-质谱法(GC-MS)评估了γ-PGA对地衣芽孢杆菌胞内代谢物检测的影响。结果表明:终浓度为10g/L的γ-PGA会使除谷氨酸外的氨基酸、有机酸和糖类标准品的检测峰面积明显降低;另外,终浓度为10g/L的γ-PGA对分析地衣芽孢杆菌胞内代谢物中的谷氨酸、葡萄糖和6-磷酸葡萄糖(G6P)的影响较为显著。因此,在利用GC-MS定量分析地衣芽孢杆菌的胞内代谢物前,应将γ-PGA和细胞分离。
In this work, we assessed the effect of exogenous poly-γ-glutamate on the detection of intracellular metabolites in Bacillus licheniformis by gas chromatography-mass spectrometry（GC-MS）. The results indicated that 10 g/L γ-PGA added in standard solutions obviously decreased the peak areas of amino acids,organic acids and sugars, except glutamate. For the intracellular metabolites of Bacillus licheni f ormis , the phosphate were al before quantifying effects of γ-PGA on the detection of intracellular glutamate, glucose and glucose 6- so extremely remarkable. Therefore,it is necessary to isolate γ-PGA from the broth the intracellular metabolites by GC-MS.
出处
《分析科学学报》
CAS
CSCD
北大核心
2013年第2期187-190,共4页
Journal of Analytical Science
基金
国家自然科学基金(No.31170046)
关键词
聚Γ-谷氨酸
气相色谱-质谱
地衣芽孢杆菌
代谢物
样品预处理
Poly-γ-glutamate
Gas chromatography-mass spectrometry
Bacillus licheniforrnis
Metabolites
Sample preparation
