摘要
研究针对布鲁杆菌保守的OMP25基因设计了4条LAMP的特异性引物,通过优化反应条件,建立了布鲁杆菌的LAMP快速检测方法。特异性和敏感性试验结果显示,该方法能够特异地检测布鲁杆菌,其最低检测限为5cop-ies/μL。扩增产物可通过琼脂糖凝胶电泳、显色反应进行判定。LAMP可以快速、直观、准确地检测布鲁杆菌,是一种适合基层现场应用的检测方法。
A loop-mediated isothermal amplification（LAMP） method for rapidly detecting Brucella spp was established using two sets of Brucella-specific primers according to the species-specific OMP25 gene and optimizing the reaction condition. The results of specificity and sensitivity tests showed that the method was able to detect Brucella spp specifically and the limit of detection was as low as 5 copies/μL. The amplified products were judged by agarose gel electrophoresis and chromogenic reaction. The LAMP method is suitable for field detection.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第4期576-580,共5页
Chinese Journal of Veterinary Science
基金
国家高技术研究发展计划(863计划)资助项目(2012AA101605)
天津市科技支撑计划重点资助项目(10ZCZDNC02800)
