摘要
为了研究采用ASE-100加速溶剂萃取仪提取万寿菊(Tagetes erecta L.)花中叶黄素的最佳工艺条件,采用五因素五水平正交试验提取叶黄素,用高效液相色谱法测定皂化后的叶黄素产量。用ASE-100加速溶剂萃取仪提取叶黄素的最佳试验参数为提取温度80℃、料液比1∶30(m/V,g∶mL)、静态萃取时间10 min、静态周期数3、冲洗体积70%。
To optimize the technical conditions for extracting lutein from marigold flower by ASE-100 Accelerated Solvent Extractor,five factors-five levels orthogonal design was applied.And the yeild of lutein was determined by HPLC after saponification.The optimal lutein extraction conditions from marigold flower were extraction temperature,80 ℃;solid to liquid ratio,1∶30(m/V,g∶mL);static extraction time,10 min;static cycle,3;flush volume,70%.
出处
《湖北农业科学》
北大核心
2012年第18期4094-4096,4108,共4页
Hubei Agricultural Sciences