摘要
目的研究蚓激酶对肺癌细胞A549的增殖及细胞周期的影响,初步探讨蚓激酶抗肺癌的作用机制。方法通过MTT法检测不同浓度的蚓激酶作用于A549细胞24 h后对细胞增殖的影响;通过显微镜观察细胞形态变化;流式细胞仪检测蚓激酶对A549的细胞周期阻滞情况;RT-PCR检测蚓激酶对P53基因表达影响。结果蚓激酶对A549有明显抑制作用,且抑制作用呈浓度依赖性,其半数抑制浓度(IC50)为(54.35±2.112)U/mL;A549经蚓激酶作用后形态变化显著,细胞边缘皱缩,漂浮细胞的数目随着药物浓度的增高而增大;蚓激酶阻滞A549细胞周期于S期;RT-PCR检测表明,蚓激酶能明显上调P53基因的表达。结论蚓激酶在体外能明显抑制肺癌细胞A549的增殖,并阻滞细胞周期于S期,其机制可能与上调P53基因表达有关。
Objective To investigate the effect and mechanism of lumbrokinase on the proliferation and cycle of lung cancer cells A549.Methods MTT assay was used to determine the proliferation of A549 cells treated with different concentrations of lumbrokinase for 24 h.The change of cell morphology was observed by microscopy.The alteration of cell cycle was determined by flow cytometry.The expression of P53 gene was measured by RT-PCR.Results Lumbrokinase inhibited the proliferation of A549 cells with a dose dependent manner and the half inhibitory concentration（IC50） was（54.35±2.112） U/mL.After treated with lumbrokinase,the cell morphology changed significantly and the percent of floating cells enhanced.Meantime,lumbrokinase arrested the cell cycle in S-phase and up-regulated the expression of P53 gene.Conclusion Lumbrokinase can inhibit the proliferation of lung cancer cells A549 and arrest cell cycle in S-phase.Its anti-tumor mechanism might be related to the up-regulation of P53 expression.
出处
《广东药学院学报》
CAS
2013年第1期79-82,共4页
Academic Journal of Guangdong College of Pharmacy
基金
国家自然科学青年基金项目(30901114)
