摘要
目的:建立糖尿病易感基因FTO中三个变异位点的非标记探针(LunaProbe)检出方法,初步进行临床应用。方法:基于高分辨率熔解曲线(HRM)技术,通过控制PCR产物长度和非标记寡核苷酸探针的特异性设计,通过Idaho LightScanner分析,建立FTO基因rs8050136、rs9939609和rs9930506的LunaProbe基因分型技术,并在2型糖尿病患者群体中进行分型应用。结果:所建立的基于LunaProbe的三个变异位点的分型方法,不受限于基因变异的位置特征,可成功区分三个变异位点的不同突变型和野生型,并且在糖尿病患者人群中可达到较高的重复性和准确性。结论:LunaProbe的HRM分型方法,可成功对糖尿易感基因FTO的三个变异位点rs8050136、rs9939609和rs9930506进行人群分型,且具有敏感、经济、高通量的特性。
Objective：To set up a high resolution melting analysis based LunaProbe genotyping method for the detection of variations of FTO gene in T2DM patients. Methods： Based on the high resolution melting theory, the length of PCR products was set and the unlabeled oligonucleotides probe was designed. Then the genotyping technology for rs8050136, rs9939609 and rs9930506 in FTO gene was established with LunaProbe after Idaho LightScanner analysis, and applied to genotype in T2DM patients. Results： According to the genotyping methods based on LunaProbe analysis of these 3 variations, each genotype was successfully distinguished without limitation of the base position, and it still has good performance when used in the practice of T2DM population. Conclusion： The LunaProbe genotyping method based on HRM could be successfully used in the detection of variation of FTO gene in T2DM patients as a sensitive, costeffective and relative high- throughput method.
出处
《中国卫生检验杂志》
北大核心
2013年第3期529-531,共3页
Chinese Journal of Health Laboratory Technology
基金
北京市科技新星计划(Z121107002512058)
国家自然科学基金中加合作项目(81010052)
北京医院重大基金(BJ-2010-30)
