摘要
以补硒栽培的水稻为材料制备水溶性蛋白 ,运用 ( NH3) 2 SO4分段盐析、分子筛柱层析 ,分离出高含硒蛋白组分 ,经酸水解后采用标样对照法 ,用高效液相色谱分离、检出硒代氨基酸 ,再用质谱法进一步确认 .在水稻硒蛋白中 ,硒的结合形态 ,硒代蛋氨酸和硒代光胱氨酸两种均存在 .
Soluble proteins were extracted from rice grown. with added selenium. The proteins riched with selenium were separated by (NH 4) 2SO 4 fractional salting out and molecular sieve column chromatography. The Se amino acids were separated and determined with HPLC by a trade sample contrast after hydrolyzed, which were affirmed more by mass spectroscopy.In rice se proteins, there are both seleno methionine and seleno cyteine for the combined forms of selenium.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第2期223-225,共3页
Journal of Central China Normal University：Natural Sciences
基金
湖北省教委重点资助项目 !( 97A2 4 )
