摘要
目的观察大鼠模拟海训皮肤擦伤经海水浸泡后炎症因子的变化,探讨医用贴膜对皮肤擦伤的保护作用。方法 40只雄性SD大鼠,分为擦伤未浸泡组(A组),擦伤蒸馏水浸泡组(B组),擦伤海水浸泡组(C组),擦伤贴膜保护海水浸泡组(D组)及无擦伤不浸泡对照组(E组),每组8只。除E组外其余各组均以消毒工业砂纸在大鼠右后肢皮肤进行擦伤处理。A组未予以浸泡处置,B组浸泡于蒸馏水0.5 h,C组浸泡于海水0.5 h,D组擦伤后用医用贴膜敷贴保护创面,海水浸泡0.5 h。A、B、C、D四组分别于擦伤后0.5、6、12、72 h取尾静脉血,E组在0.5 h时取血,用酶联免疫测定(ELISA)方法测定血清IL-1β、IL-6、TNF-α含量,比较各组之间的差异,取病变皮肤行病理组织学检查。结果 A、B、C、D四组三种炎症因子水平在0.5、6、12 h时相点均明显高于E组(P<0.01),A、B、C、D四组在72 h时相点无统计学差异(P>0.05);与C组比较,B组的三种因子在12 h时相点均显著降低(P<0.01),D组的IL-1β、TNF-α在12 h时相点也显著降低(P<0.05)。除12 h时相点的IL-6外,B、D两组其他时相点的三种因子水平均无统计学差异(P>0.05)。结论大鼠皮肤擦伤经海水浸泡后炎症因子水平显著升高,局部炎症加重;医用贴膜敷贴擦伤创面后,炎症因子水平相对降低,局部炎症减轻,医用贴膜有一定的保护作用。
Objective To observe the changes in inflammatory factors of rats undergoing skin abrasion after sea water immersion and evaluate the application of medical drapes to abrasion during sea training. Methods Forty male SD rats were randomly as- signed to five groups with eight each : abrasion without immersion ( A), abrasion with distilled water immersion ( B), abrasion with sea water immersion ( C), abrasion with medical drapes plus sea water immersion (D) and controls (E). The rats' right lateral limb was abraded by industrial sandpaper except those in Group E. The immersing time was 0.5h for Group B, C and D. Steri- Drape was used on the skin abrasion in Group D. Then the blood samples were extracted from tail vein after 0.5, 6, 12 and 72h. With ELISA method, the concentrations of three kinds of inflammatory factors (IL-1β, IL-6,TNF-α)were assayed. And pathological examination was performed. Results The concentrations of the three inflammatory factors of Group A, B, C and D were higher than that of Group E significantly (P 〈 0.01 ) , and there were no significant differences at the time point of 72h among the former four groups (P 〉 0.05). Compared with Group C, the concentrations of the three inflammatory factors of group B were lower at the time point of 12h (P 〈 0.01 ) , and so were that of IL-1β and TNF-α of Group D at the same time point (P 〈 0.05). The concen- trations of the three inflammatory factors of group B and D were of no diffidence except IL-6 at the time point of 12h (P 〉0.05 ). Conclusion Inflammatory factors of the rats with skin abraded increase after sea water immersion with a severe local inflammation. With Steri-Drape on the surface of skin abrasion, inflammatory factors decrease with a relatively slight local inflammation. Steri- Drape has some effect of protection on skin abrasion against sea water immersion.
出处
《临床军医杂志》
CAS
2013年第3期219-221,共3页
Clinical Journal of Medical Officers
关键词
海水
炎症因子
皮肤擦伤
海训
seawater
inflammatory factor
skin abrasion
sea training