摘要
痰或其它各种体液内结核分枝杆菌（MTB）阳性是诊断结核病（TB）的金标准.结核硬脂酸（TBSA）是鉴定分枝杆菌与其它非分枝杆菌的特征组分,可用于TB的诊断[1,2]。
Two kinds of methods were used to prepare basic fuchsin （BF） modified magnetic nanoparticles The obtained BF modified nanoparticles （MNP-1 and MNP-2） were characterized using transmission electron microscope （ TEM ） and Fourier transformation infrared （FTIR） um from Staphylococcus aureus and Escherichia coli （E. coli）. tion of bacteria, temperature, ionic strength and adsorbent studied. The results show that both MNP-1 and MNP-2 have spectroscopy before used to adsorb mycobacteri- The effects of pH, adsorption time, concentra- dosage on the adsorption of bacterium were good selectivity for mycobacterium, and can be easily separated by external magnetic field. Under the optimized conditions, the highest adsorption rate for mycobacteria were 65% （ MNP-1 ） and 85% （ MNP-2）, while only 20% of Staphylococcus aureus and 10% of E. coli can be adsorbed under the same conditions. The adsorption capacity of MNP-1 and MNP-2 for myco- bacteria was 1.48×10^10 and 3.25×10^10 cfu/g, respectively.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2013年第4期760-762,共3页
Chemical Journal of Chinese Universities
基金
湖北省教育厅指导性科研计划项目(批准号:B20110809)
中南民族大学学术团队科研基金项目(批准号:XTZ09005)资助
关键词
磁性微球
碱性品红
细菌分离
分枝杆菌
Magnetic microspheres
Basic fuchsin
Bacterium separation
Mycobacterium
