摘要
产电微生物是驱动微生物燃料电池(Microbial fuel cell,MFC)运行的关键.采用分离培养-MFC产电实验的方法,从以污水处理厂活性污泥为接种物的MFC阳极生物膜中分离到一株产电微生物,命名为Z6.菌株Z6的16S rDNA序列与已知菌株Klebsiell aoxytoca An16-2具有100%的同源性,结合该菌生理和形态特征将其初步鉴定为克雷伯氏菌属.菌株Z6接种MFC的产电结果表明,以柠檬酸钠为底物时MFC的最大体积功率密度达到14.35W·m-3,内阻为400Ω,显示出较强的电化学活性.通过循环伏安分析和外源AQDS添加实验结果推测,菌株Z6很可能通过分泌电子传递中间体传递电子.
Electrogens is key to boost a microbial fuel cell （MFC）. Based on isolation-MFC electricity-generating experiment method, an electrogenic bacterium was isolated from the anodic biofilm of a MFC that was inoculated with aerobic sewage sludge. The electrogenic bacterium was labeled as Z6. The 16S rDNA sequence of Z6 had 100% similarity with known strain Klebsiella oxytoca An16-2. By the consideration of physiological and morphological characteristics, the strain Z6 was preliminarily identified as a member of Klebsiella genus. The electricity-generating results of the MFC inoculated with strain Z6 showed that the maximum power density and internal resistance was 14.35 W·m^-3 and 400 Ω, respectively, when citrate was used as the substrate. It was demonstrated that strain Z6 had a relative high electrochemical activity. It can be inferred from the results of cyclic voltammetry analysis and exo-AQDS adding experiment that strain Z6 may transfer electrons through excreting mediators.
出处
《环境科学学报》
CAS
CSCD
北大核心
2013年第4期1035-1042,共8页
Acta Scientiae Circumstantiae
基金
国家自然科学基金(No.30800796
31272482)
教育部新世纪优秀人才支持计划(No.NCET-11-0166)
教育部高校博士点专项基金(No.20070561082)
广东省科技计划项目(No.2007A020100001)
中央高校基本科研业务费重点项目(No.2009zz0073)~~
