摘要
目的建立葛根与粉葛质量比较的色谱方法,并用于二者的质量分析与评价,为其质量控制提供新依据,为其药效物质基础的分析奠定基础。方法采用薄层色谱(TLC)法对7个葛根样品和17个粉葛样品进行比较,并采用高效液相色谱(HPLC)法进行指纹图谱测定,运用中药色谱指纹图谱相似度评价系统(2004A)软件进行分析。采用对照品对比和HPLC-MS技术对其部分色谱峰进行定性鉴别。结果建立的TLC和HPLC指纹图谱能区别葛根和粉葛。HPLC指纹图谱粉葛饮片共有峰14个,野葛根饮片共有峰19个,初步指认了葛根与粉葛HPLC指纹图谱中13和7个成分。结论该文为葛根与粉葛质量控制和临床应用提供可靠依据。
Objective To establish chromatographic fingerprints for the identification and quality evaluation of Pueraria lobata and Pueraria thomsonii. Methods Seventeen samples of Pueraria thomsonii and 7 samples of Puerar/a lobata were analyzed with the thin-layer chromatography （TLC） and the developed high performance liquid chromatography （HPLC） fingerprint method. The HPLC data was calculated with similarity evaluation system for chromatographic fingerprint of TCM （ Version 2004A）. Results A satisfactory fingerprint was obtained by HPLC method, and qualitative analyses of some of the peaks were carried out by HPLC/MS approach. Conclusion The present research provides useful evidence for the quality control of evaluation of Pueraria lobata and Pueraria thomsonii.
出处
《医药导报》
CAS
北大核心
2013年第4期525-529,共5页
Herald of Medicine
关键词
葛根
粉葛
色谱指纹图谱
Pueraria lobata
Pueraria thomsonii
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