摘要
目的探讨采用精子透明带结合法优选的精子进行卵胞浆内单精子注射(ICSI)的临床意义。方法选取2010年6月至2012年2月因男方因素在本中心行ICSI治疗的夫妇54个周期,随机将患者分为研究组和对照组,各27例,研究组患者采用与透明带结合的精子进行ICSI,对照组采用常规方法进行ICSI,比较两组患者的受精率、卵裂率、优胚率及着床率。结果研究组与对照组女方年龄、不育年限、促性腺激素(Gn)支数、MII卵母细胞数、移植胚胎数目无显著性差异(P>0.05);两组临床妊娠率(59.26%vs.48.15%),研究组高于对照组,但无显著性差异(P>0.05);两组的受精率(82.97%vs.81.63%)、卵裂率(96.32%vs.96.50%)无显著性差异(P>0.05);优胚率(66.12%vs.50.78%)和可利用胚胎率(75.95%vs.66.32%)均显著高于对照组(P<0.05)。结论通过采用患者自体卵母细胞透明带结合的方法选择精子进行ICSI可以提高胚胎质量,增加可利用胚胎的数量,有助于改善临床妊娠结局。
Objective： To investigate whether selection of sperm which can bind to zona pellucida（ZP）for intracy- toplasmic sperm injection（ICSI）could improve clinical pregnancy. Methods： A total of 54 ICSI patients were randomly divided into study group（27 patients）and control group（27 patients）according to using ZP-bound sperm or not. The ZP-bound sperms were prepared by using sibling immature oocytes. Results： The fertilization rate was similar between the two groups（82.97% vs. 81.63 %）（P：〉0.05）. The proportion of high quality embryos（66.12% vs. 50.78%）was significantly higher in study group than that in control group（P〈0.05）. The clinical pregnancy was better in study group than that in control group（59.26% vs. 48.15%）,but it was not significant. Conclusions： Selection of ZP-bound sperm for ICSI may improve embryo quality and implantation rate. This may be a new way for ICSI.
出处
《生殖医学杂志》
CAS
2013年第3期155-158,共4页
Journal of Reproductive Medicine
基金
2011年银川市科技攻关项目(201140)
