摘要
背景:富血小板血浆是目前已知富含多种生长因子并能将其释放的自体提取物,并已应用于骨、软骨组织工程再生的研究。目的:通过比较不同方法制备兔富血小板血浆中血小板浓度,并测定其血小板源性生长因子、转化生因子β1水平,探讨富血小板血浆制备方法及影响因素。方法:采用Petrungaro法、Landesberg法、Aghaloo法制备新西兰大耳白兔富血小板血浆。检测3组富血小板血浆中血小板计数,以及3组富血小板血浆活化前后及正常血浆、贫血小板血浆中血小板源性生长因子、转化生因子β1水平。结果与结论:3种方法制备的富血小板血浆中血小板计数、血小板回收率、血小板富集系数差异有非常显著性意义(P<0.001),Landesberg法和Aghaloo法均可制备有效浓度的富血小板血浆,且Aghaloo法制备血小板浓度及活性高于Landesberg法(P<0.05)。活化前3组富血小板血浆中血小板源性生长因子、转化生因子β1水平与正常血浆组、贫血小板血浆组比较差异无显著性意义。活化后,Landesberg法和Aghaloo法制备的血小板血浆中血小板源性生长因子、转化生因子β1水平明显高于活化前(P<0.001),且Aghaloo法最高(P<0.05)。富血小板血浆中血小板计数与血小板源性生长因子水平(r=0.872,P<0.001),转化生因子β1水平(r=0.917,P<0.001)呈正相关。
BACKGROUND： Platelet-rich plasma is an autogeneic extract containing and releasing a variety of growth factors, which has been used in the research of bone and cartilage tissue engineedng and regeneration. OBJECTIVE： To investigate the preparation methods of platelet-rich plasma and its influencing factors by comparing the platelet concentration in the platelet-rich plasma prepared by different methods in rabbits and detecting the levels of platelet-derived growth factor and transforming growth factor β1. METHODS： Platelet-rich plasma was prepared by the methods of Petrungaro, Landesberg and Aghaloo. Platelet amount was counted in the platelet-rich plasma prepared using three methods as well as platelet-derived growth factor and transforming growth factor β1 levels. RESULTS AND CONCLUSION： There were significant differences in the platelet count, platelet recovery rate and platelet enrichment coefficient in the platelet-rich plasma prepared by these three methods （P 〈 0.001 ）. The effective concentration of platelet-rich plasma could be prepared by the methods of Landesberg and Aghaloo, and the platelet concentration and activity prepared by Aghaloo method was higher than that prepared by Landesberg method with a statistical difference （P 〈 0.05）, The levels of platelet-derived growth factor and transforming growth factor β1 in the inactivated platelet-rich plasma were not statistically different from those in the normal plasma and platelet-poor plasma. The levels of platelet-derived growth factor and transforming growth factor β1 in the activated platelet-rich plasma prepared by Landesberg and Aghaloo methods were significantly higher than before activation （P 〈 0.001 ）, especially in the activated platelet-dch plasma prepared by Aghaloo method （P 〈 0.05）. The platelet count in the piatelet-rich plasma was positively correlated with the levels of platelet-derived growth factor （r=0.872, P 〈 0.001 ） and transforming growth factor β1 （r=0.917, P 〈 0.001 ）.
出处
《中国组织工程研究》
CAS
CSCD
2013年第8期1411-1417,共7页
Chinese Journal of Tissue Engineering Research
基金
天津市卫生局科技基金重点攻关课题(KG109)~~
关键词
生物材料
生物材料基础实验
富血小板血浆
血浆
血小板
血小板源性生长因子
转化生长因子
血小板活化
软骨组织
活化
组织工程
组织再生
其他基金
生物材料图片文章
biomaterials
basic experiments of biomaterials
platelet-rich plasma
plasma
platelet
platelet-derived growth factor
transforming growth factor
platelet activation
cartilage
activation
tissueengineering
tissue regeneration
other grants-supported paper
biomaterial photographscontaining paper
