摘要
为了更好地理解镉对人体的健康效应以保障人体健康,以典型天然雌激素雌二醇(E2)为代表,研究了E2与Cd的共同作用效应.主要研究了人体乳腺癌细胞MCF-7增殖、周期分析、雌激素活性作用途径等.结果发现Cd具有雌激素活性,但与E2共存时发生拮抗作用,雌激素活性明显下降.细胞周期研究表明,E2,Cd及两者混合物均能提高DNA合成期细胞比例,促进细胞增殖.增殖途径分析表明,E2,Cd及其混合物使细胞内雌激素受体α(ERα)蛋白的表达量明显降低,拮抗作用的发生主要来自丝裂原活化蛋白激酶(MAPK)信号转导通路.E2和Cd可以诱导增殖相关蛋白Ki67的表达,促进细胞增殖,而E2与Cd联合使Ki67的表达极大地降低.
17-Estradiol （E2）, a typical natural estrin, was chosen to study the mixed effect of Cd with natural estrins. The aim is to understand the impact of Cd on human health. Human breast cancer cell MCF-7 increase examination was used to examine the estrogenicity. The results showed that Cd had estro- gencity, but E2 dramatically decreased its effect. Cell cycle analyses showed that Cd, E2, and their mix- ture all increased the ratio of cells in the DNA synthetic phase and therefore promoted cell proliferation. Cell proliferation pathway analyses showed that Cd, E2, and mixture significantly reduced the expression of ERa protein in the cell. The rivalry effect between E2 and Cd was mainly from the MAPK signal trans- duction pathway. E2 and Cd both promoted the expression of Ki67 protein and prolification-related pro- tein, but the mixture significantly reduced the expression of Ki67.
出处
《湖南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2013年第3期83-86,共4页
Journal of Hunan University:Natural Sciences
基金
国家自然科学基金资助项目(51178047)
芙蓉学者计划项目(湘教办通[2010]293-10)
