摘要
对衍生于普通小麦与八倍体小偃麦‘小偃7430’杂种后代的抗条锈病新种质CH7102进行抗性鉴定和遗传分析,明确其抗性来源及其遗传方式。采用条锈菌流行小种CYR31、CYR32对CH7102及其亲本进行苗期抗性评价;对CH7102分别与感病品种和已知抗性基因载体品系的杂交后代接种CYR32进行成株期抗条锈性遗传分析和等位性测验。CH7102具有与其抗病亲本‘小偃7430’和彭提卡偃麦草相似的侵染型,而所有的小麦亲本均感病,表明CH7102的抗性来自彭提卡偃麦草;CH7102与感病品种‘台长29’和‘绵阳11’杂交、回交,其F2、BC1、F2:3代的抗、感分离比分别符合3:1、1:1和1:2:1的单显性基因分离模式。而CH7102与已知抗性基因载体品系杂交F2代的抗感分离比为15:1。CH7102对条锈病的抗性来自彭提卡偃麦草,其抗性受1对显性核基因控制,而且与已知的抗CYR31、CYR32的抗性基因Yr5、Yr10、Yr15、Yr24/Yr26、Yr41不存在等位关系,属新的抗条锈病基因。
This study aimed at determining the response to Chinese stripe rust races, the origin and inheritance mode of resistance gene in a new Thinopyrum ponticum-derived wheat introgression line CH7102. Seedlings of Th. ponticum （accession R431）, CH7102 and its resistant parent partial amphiploid‘Xiaoyan 7430’as well as all wheat parents were evaluated in the greenhouse using CYR31 and CYR32, the most widely virulent and predominant pathotypes in China. CH7102 was crossed respectively to susceptible cultivars‘Taichung 29’ and‘Mianyang 11’and the lines containing known resistance genes, and the resulting segregating populations F2, F3 and BC1 were used for genetic analysis of stripe rust response and allelism tests of resistance gene in CH7102. When inoculated with race CYR32 at the adult plant stage, F1 plants from both crosses showed infection types （IT） similar to the resistant parent, indicating that resistance was dominant. The segregation ratios in the F2 and BC1 populations and F3 lines were consistent with those expected for segregation at a single locus. When tested with the same race at the seedling stage, the segregation for resistant and susceptible showed a good fit with a 15：1 ratio in all F2 populations from crossing CH7102 with five lines containing known resistance genes. The stripe rust resistance in wheat-alien introgression line CH7102 was controlled by a single dominant gene, presumably having been transferred from T. ponticum. Based on the result from allelism tests, the resistance gene in CH7102 was different from the known effective resistance genes Yr5, Yr10, Yr15 Yr24/ Yr26 and Yr41, and is possibly a new gene for resistance to stripe rust.
出处
《中国农学通报》
CSCD
2013年第9期51-56,共6页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"兼抗白粉病和条锈病小偃麦渗入系创制
分子鉴定及抗病基因标记定位"(31171839)
山西省回国留学人员科研项目"抗条锈病小偃麦新抗源创制及抗病新基因分子鉴定"(2012-102)
山西省国际科技合作计划"小麦白粉病新抗源创制及其抗病新基因鉴定"(2012081006-2)
